Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1 antibody at 1:200 dilution.)
Rabbit MAF1 Polyclonal Antibody | anti-MAF1 antibody
MAF1 antibody
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
MAF1 antibody was purified by antigen-affinity chromatography
Synonyms
MAF1; Polyclonal Antibody; MAF1 antibody; Polyclonal MAF1; Anti-MAF1; MGC39758; MAF1 homolog (S. cerevisiae); repressor of RNA polymerase III transcription MAF1 homolog; MGC20332; MAF-1; MGC31779; homolog of yeast MAF1; MAF 1; anti-MAF1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
MAF1 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
256
Applicable Applications for anti-MAF1 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
IF:1:100-1:200
WB: 1:500-1:3000
WB: 1:500-1:3000
Biological Significance
This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III. It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.
Cross-Reactivity
Human
Immunogen
MAF1 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 161 of MAF1
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1 antibody at 1:200 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug whole cell lysate (A:A431; B:MOLT4) using a 12 % SDS PAGE gel and MAF1 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug whole cell lysate (A:A431; B:MOLT4) using a 12 % SDS PAGE gel and MAF1 antibody at a dilution of 1:1000)
Related Product Information for anti-MAF1 antibody
Affinity purified Rabbit polyclonal MAF1 antibody
Product Categories/Family for anti-MAF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
29 kDa
NCBI Official Full Name
MAF1
UniProt Protein Name
Repressor of RNA polymerase III transcription MAF1 homolog
Protein Family
UniProt Gene Name
MAF1
UniProt Entry Name
MAF1_HUMAN
Uniprot Description
MAF1: Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA. Belongs to the MAF1 family.
Protein type: Transcription regulation
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: nucleoplasm; centrosome; intracellular membrane-bound organelle; axon; cytoplasm; dendrite; plasma membrane; nucleolus; intracellular; nucleus; cytosol
Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase III promoter
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Product Notes
The MAF1 maf1 (Catalog #AAA837408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MAF1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF:1:100-1:200 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the MAF1 maf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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