Mouse Dexamethasone-induced Ras-related protein 1 ELISA Kit | Rasd1 elisa kit
Mouse Dexamethasone-induced Ras-related protein 1 ELISA Kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Ras superfamily of small GTPases and is induced by dexamethasone. The encoded protein is an activator of G-protein signaling and acts as a direct nucleotide exchange factor for Gi-Go proteins. This gene may play a role in dexamethasone-induced alterations in cell morphology, growth and cell-extracellular matrix interactions. [provided by RefSeq, Nov 2015]
Uniprot Description
RASD1: Small GTPase. Negatively regulates the transcription regulation activity of the APBB1/FE65-APP complex via its interaction with APBB1/FE65. Forms a ternary complex with CAPON and NOS1. Component of a complex, at least composed of APBB1, RASD1/DEXRAS1 and APP. Interacts with APBB1/FE65. By dexamethasone. Expressed in a variety of tissues including heart, cardiovascular tissues, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, gastrointestinal and reproductive tissues. Belongs to the small GTPase superfamily. RasD family.
Protein type: G protein; G protein, monomeric; G protein, monomeric, RasD
Cellular Component: cytoplasm; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; sarcoplasmic reticulum
Molecular Function: GTP binding; nucleotide binding; protein binding
Biological Process: negative regulation of transcription, DNA-dependent; nitric oxide mediated signal transduction; signal transduction; small GTPase mediated signal transduction
Research Articles on Rasd1
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Product Notes
The Mouse Rasd1 rasd1 (Catalog #AAA2880442) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2880442 ELISA Kit recognizes Mouse Rasd1. It is sometimes possible for the material contained within the vial of "Dexamethasone-induced Ras-related protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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