Rabbit anti-Human HNRNPC Polyclonal Antibody | anti-HNRNPC antibody

HNRNPC, CT (HNRNPC, HNRPC, Heterogeneous nuclear ribonucleoproteins C1/C2) (Azide free) (HRP)

Cat. Num. AAA6307466

• Gene Names: HNRNPC; C1; C2; HNRNP; HNRPC; SNRPC
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HNRNPC; Polyclonal Antibody; CT (HNRNPC; HNRPC; Heterogeneous nuclear ribonucleoproteins C1/C2) (Azide free) (HRP); Heterogeneous nuclear ribonucleoproteins C1/C2; anti-HNRNPC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HNRNPC antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HNRNPC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-310 amino acids from the C-terminal region of human HNRNPC.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(HNRNPC antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HNRNPC antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(HNRNPC Antibody (C-term) western blot analysis in Jurkat,K562,U251 cell line lysates (35ug/lane).This demonstrates the HNRNPC antibody detected the HNRNPC protein (arrow).)

Related Product Information for anti-HNRNPC antibody

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq].

Product Categories/Family for anti-HNRNPC antibody

Antibodies; Nuclear Proteins

References

Konig, J., et al. Nat. Struct. Mol. Biol. 17(7):909-915(2010). Lee, E.K., et al. Nat. Struct. Mol. Biol. 17(6):732-739(2010). Brunner, J.E., et al. Virology 400(2):240-247(2010). Ertel, K.J., et al. J. Virol. 84(9):4229-4242(2010). Mosessian, S., et al. J. Biol. Chem. 284(44):30159-30166(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 117190174
• NCBI GeneID: 3183
• NCBI Accession #: NP_004491.2
• NCBI GenBank Nucleotide #: NM_004500.3
• UniProt Accession #: P07910
• NCBI Official Full Name: heterogeneous nuclear ribonucleoproteins C1/C2 isoform b
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein C
• NCBI Official Symbol: HNRNPC
• NCBI Official Synonym Symbols: C1; C2; HNRNP; HNRPC; SNRPC
• NCBI Protein Information: heterogeneous nuclear ribonucleoproteins C1/C2
• UniProt Protein Name: Heterogeneous nuclear ribonucleoproteins C1/C2
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPC
• UniProt Synonym Gene Names: HNRPC; hnRNP C1/C2
• UniProt Entry Name: HNRPC_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP C1/C2: a heterogeneous nuclear ribonucleoprotein. May play a role in ribonucleosome assembly by neutralizing basic proteins such as A and B core hnRNP. Contains 1 RNA recognition motif (RRM) domain. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; spliceosome; protein complex; membrane; nuclear chromatin; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; nucleosomal DNA binding; mRNA 3'-UTR binding; RNA binding; nucleotide binding; poly(U) binding

Biological Process: nuclear mRNA splicing, via spliceosome; osteoblast differentiation; RNA splicing; ATP-dependent chromatin remodeling; gene expression