Mouse Transferrin receptor protein 2 ELISA Kit | Tfr2 elisa kit

Mouse Transferrin receptor protein 2 ELISA Kit

Cat. Num. AAA2882865

• Gene Names: Tfr2; Trfr2
• Reactivity: Mouse
• Synonyms: Transferrin receptor protein 2; Mouse Transferrin receptor protein 2 ELISA Kit; TfR2; Tfr2; Trfr2; Tfr2 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse Transferrin receptor protein 2,TfR2
• Sequence Length: 842

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.78-50 ng/mL
• Sensitivity: < 0.39 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.5%
• Inter-assay Precision: Inter-Assay CV: <=7.9%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Tfr2 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 575501601
• NCBI GeneID: 50765
• NCBI Accession #: NP_001276436.1
• NCBI GenBank Nucleotide #: NM_001289507.1
• UniProt Accession #: Q9JKX3; Q920I6; Q99MQ9; Q9CPT2
• Molecular Weight: 79,255 Da
• NCBI Official Full Name: transferrin receptor protein 2 isoform a
• NCBI Official Synonym Full Names: transferrin receptor 2
• NCBI Official Symbol: Tfr2
• NCBI Official Synonym Symbols: Trfr2
• NCBI Protein Information: transferrin receptor protein 2
• UniProt Protein Name: Transferrin receptor protein 2
• Protein Family: Transferrin receptor protein
• UniProt Gene Name: Tfr2
• UniProt Synonym Gene Names: Trfr2; TfR2
• UniProt Entry Name: TFR2_MOUSE

Uniprot Description

TFR2: Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation. Defects in TFR2 are a cause of hemochromatosis type 3 (HFE3). HFE3 is a disorder of iron hemostasis resulting in iron overload and has a phenotype indistinguishable from that of hereditary hemochromatosis (HH). HH is characterized by abnormal intestinal iron absorption and progressive increase of total body iron, which results in midlife in clinical complications including cirrhosis, cardiopathy, diabetes, endocrine dysfunctions, arthropathy, and susceptibility to liver cancer. Since the disease complications can be effectively prevented by regular phlebotomies, early diagnosis is most important to provide a normal life expectancy to the affected subjects. Belongs to the peptidase M28 family. M28B subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: cytoplasm; cytoplasmic vesicle; external side of plasma membrane; integral to membrane; integral to plasma membrane; membrane; plasma membrane

Molecular Function: glycoprotein binding; transferrin receptor activity

Biological Process: cellular iron ion homeostasis; iron ion homeostasis; positive regulation of endocytosis; positive regulation of transcription from RNA polymerase II promoter; receptor-mediated endocytosis; response to iron ion; transferrin transport