Human Dexamethasone-Induced Ras-Related Protein 1 (RASD1) RTU ELISA Kit | RASD1 rtu elisa kit

Human Dexamethasone-Induced Ras-Related Protein 1 (RASD1) ELISA Kit

Cat. Num. AAA4503893

• Gene Names: RASD1; AGS1; DEXRAS1; MGC:26290
• Reactivity: Human
• Synonyms: Dexamethasone-Induced Ras-Related Protein 1 (RASD1); Human Dexamethasone-Induced Ras-Related Protein 1 (RASD1) ELISA Kit; Activator of G-protein signaling 1; RASD1 rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RASD1. No significant cross-reactivity or interference between RASD1 and analogues was observed.
• Sequence Length: 122

• Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.065ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RASD1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RASD1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RASD1 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of RASD1 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RASD1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RASD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RASD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RASD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 317008582
• NCBI GeneID: 51655
• NCBI Accession #: NP_001186918.1
• NCBI GenBank Nucleotide #: NP_001186918.1
• UniProt Accession #: Q9Y272
• NCBI Official Full Name: dexamethasone-induced Ras-related protein 1 isoform 2
• NCBI Official Synonym Full Names: ras related dexamethasone induced 1
• NCBI Official Symbol: RASD1
• NCBI Official Synonym Symbols: AGS1; DEXRAS1; MGC:26290
• NCBI Protein Information: dexamethasone-induced Ras-related protein 1
• UniProt Protein Name: Dexamethasone-induced Ras-related protein 1
• Protein Family: Dexamethasone-induced Ras-related protein
• UniProt Gene Name: RASD1
• UniProt Synonym Gene Names: AGS1; DEXRAS1
• UniProt Entry Name: RASD1_HUMAN

NCBI Description

This gene encodes a member of the Ras superfamily of small GTPases and is induced by dexamethasone. The encoded protein is an activator of G-protein signaling and acts as a direct nucleotide exchange factor for Gi-Go proteins. This protein interacts with the neuronal nitric oxide adaptor protein CAPON, and a nuclear adaptor protein FE65, which interacts with the Alzheimer's disease amyloid precursor protein. This gene may play a role in dexamethasone-induced alterations in cell morphology, growth and cell-extracellular matrix interactions. Epigenetic inactivation of this gene is closely correlated with resistance to dexamethasone in multiple myeloma cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2011]

Uniprot Description

RASD1: Small GTPase. Negatively regulates the transcription regulation activity of the APBB1/FE65-APP complex via its interaction with APBB1/FE65. Forms a ternary complex with CAPON and NOS1. Component of a complex, at least composed of APBB1, RASD1/DEXRAS1 and APP. Interacts with APBB1/FE65. By dexamethasone. Expressed in a variety of tissues including heart, cardiovascular tissues, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, gastrointestinal and reproductive tissues. Belongs to the small GTPase superfamily. RasD family.

Protein type: G protein, monomeric; G protein, monomeric, RasD; G protein

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleus; perinuclear region of cytoplasm; plasma membrane; sarcoplasmic reticulum

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of transcription, DNA-dependent; nitric oxide mediated signal transduction; signal transduction; small GTPase mediated signal transduction