Human relaxin 1 ELISA Kit | RLN1 elisa kit

Human Relaxin, RLN ELISA Kit

Cat. Num. AAA702180

• Gene Names: RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• Reactivity: Human
• Synonyms: relaxin 1; Human Relaxin; RLN ELISA Kit; H1; RLXH1; bA12D24.3.1; bA12D24.3.2; preprorelaxin H1; prorelaxin; relaxin H1; RLN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human relaxin. No significant cross-reactivity or interference between human relaxin and analogues was observed.
• Sequence Length: 185

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 6.25 pg/ml -400 pg/ml
• Sensitivity: Typically less than 1.56 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RLN1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for relaxin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any relaxin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for relaxin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of relaxin bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5902052
• NCBI GeneID: 6013
• NCBI Accession #: NP_008842.1
• NCBI GenBank Nucleotide #: NM_006911.3
• UniProt Accession #: P04808; Q99936; Q9UQJ1
• Molecular Weight: 21,146 Da
• NCBI Official Full Name: prorelaxin H1 preproprotein
• NCBI Official Synonym Full Names: relaxin 1
• NCBI Official Symbol: RLN1
• NCBI Official Synonym Symbols: H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• NCBI Protein Information: prorelaxin H1; preprorelaxin H1
• UniProt Protein Name: Prorelaxin H1
• Protein Family: Prorelaxin
• UniProt Gene Name: RLN1
• UniProt Entry Name: REL1_HUMAN

NCBI Description

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The protein encoded by this gene is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. [provided by RefSeq, Jan 2013]

Uniprot Description

Function: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.