Western Blot (WB)
(Western blot detection of Proteasome subunit alpha type 6 in K562,C6,3T3,Hela cell lysates using Proteasome subunit alpha type 6 Rabbit pAb(1:1000 diluted). Predicted band size:27kDa. Observed band size:27kDa.)
Rabbit Proteasome subunit alpha type 6 Polyclonal Antibody | anti-PSMA6 antibody
Proteasome subunit alpha type 6 Rabbit pAb
ICC/IF: 1:50
FC/FACS: 1:20-1:100
Western Blot (WB)
(Western blot detection of Proteasome subunit alpha type 6 in K562,C6,3T3,Hela cell lysates using Proteasome subunit alpha type 6 Rabbit pAb(1:1000 diluted). Predicted band size:27kDa. Observed band size:27kDa.)
Western Blot (WB)
(Western blot detection of Proteasome subunit alpha type 6 in K562,C6,3T3,Hela cell lysates using Proteasome subunit alpha type 6 Rabbit pAb(1:1000 diluted). Predicted band size:27kDa. Observed band size:27kDa.)
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
NCBI and Uniprot Product Information
NCBI Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple transcript variants encoding several different isoforms have been found for this gene. A pseudogene has been identified on the Y chromosome. [provided by RefSeq, Aug 2013]
Uniprot Description
PSMA6: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Belongs to the peptidase T1A family.
Protein type: Proteasome complex; EC 3.4.25.1; Protease
Chromosomal Location of Human Ortholog: 14q13
Cellular Component: proteasome complex; nucleoplasm; polysome; proteasome core complex; sarcomere; nuclear matrix; myofibril; cytoplasm; nucleus; cytosol
Molecular Function: threonine endopeptidase activity; NF-kappaB binding; protein binding; RNA binding; endopeptidase activity
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; activation of NF-kappaB transcription factor; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of inflammatory response; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
Disease: Myocardial Infarction, Susceptibility To
Research Articles on PSMA6
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Product Notes
The PSMA6 psma6 (Catalog #AAA4752190) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Proteasome subunit alpha type 6 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Proteasome subunit alpha type 6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:2000-1:10,000 ICC/IF: 1:50 FC/FACS: 1:20-1:100. Researchers should empirically determine the suitability of the PSMA6 psma6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proteasome subunit alpha type 6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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