Mitogen Activated Protein Kinase Scaffold Protein 1 (MAPKSP1) Recombinant Protein | MAPKSP1 recombinant protein

Recombinant Mitogen Activated Protein Kinase Scaffold Protein 1 (MAPKSP1)

Cat. Num. AAA2018510

• Gene Names: LAMTOR3; MP1; MAPBP; MAPKSP1; PRO0633; MAP2K1IP1; Ragulator3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Mitogen Activated Protein Kinase Scaffold Protein 1 (MAPKSP1); Recombinant Mitogen Activated Protein Kinase Scaffold Protein 1 (MAPKSP1); MAPKSP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MADDLKRFLY KKLPSVEGLH AIVVSDRDGV PVIKVANDNA PEHALRPGFL STFALATDQG SKLGLSKNKS IICYYNTYQVVQFNRLPLVV SFIASSSANT GLIVSLEKEL APLFEELRQV VEVS
• Sequence Length: 117

• Applicable Applications for MAPKSP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Met1~Ser124 (Accession # Q9UHA4) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Late endosome membrane; Peripheral membrane protein; Cytoplasmic side
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 344179106
• NCBI GeneID: 8649
• NCBI Accession #: NP_001230665.1
• NCBI GenBank Nucleotide #: NM_001243736.1
• UniProt Accession #: Q9UHA4; Q9H364; B2R4A1; J3KMX4
• Molecular Weight: 17.4kDa
• NCBI Official Full Name: ragulator complex protein LAMTOR3 isoform 2
• NCBI Official Synonym Full Names: late endosomal/lysosomal adaptor, MAPK and MTOR activator 3
• NCBI Official Symbol: LAMTOR3
• NCBI Official Synonym Symbols: MP1; MAPBP; MAPKSP1; PRO0633; MAP2K1IP1; Ragulator3
• NCBI Protein Information: ragulator complex protein LAMTOR3; MEK binding partner 1; MAPK scaffold protein 1; mitogen-activated protein kinase scaffold protein 1; late endosomal/lysosomal adaptor and MAPK and MTOR activator 3; mitogen-activated protein kinase kinase 1 interacting protein 1
• UniProt Protein Name: Ragulator complex protein LAMTOR3
• UniProt Gene Name: LAMTOR3
• UniProt Synonym Gene Names: MAP2K1IP1; MAPKSP1; Mp1
• UniProt Entry Name: LTOR3_HUMAN

NCBI Description

This gene encodes a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Studies of the orthologous gene in mouse indicate that it regulates late endosomal traffic and cell proliferation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 13. [provided by RefSeq, Aug 2011]

Uniprot Description

LAMTOR3: Regulator of the TOR pathway, a signaling cascade that promotes cell growth in response to growth factors, energy levels, and amino acids. As part of the Ragulator complex, recruits the Rag GTPases and the mTORC1 complex to lysosomes, a key step in activation of the TOR signaling cascade by amino acids. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2. Interacts with MAP2K1/MEK1 and MAPK2. Interacts with MORG1. Part of the Ragulator complex composed of LAMTOR1, LAMTOR2 and LAMTOR3. Interacts with LAMTOR1; the interaction is direct. Belongs to the LAMTOR3 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: focal adhesion

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; protein complex scaffold

Biological Process: positive regulation of TOR signaling pathway; positive regulation of GTPase activity