Testing Data
(Western blot analysis of extracts of various celllines, using NT5E antibody.)
Rabbit NT5E Antibody | anti-NT5E antibody
NT5E Antibody
Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
NT5E; NT5E Antibody; CD73; E5NT; NT; NT5; NTE; eN; Ent; anti-NT5E antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
524
Applicable Applications for anti-NT5E antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human NT5E
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Testing Data
(Western blot analysis of extracts of various celllines, using NT5E antibody.)
Testing Data
(Western blot analysis of extracts of various celllines, using NT5E antibody.)
Related Product Information for anti-NT5E antibody
Ecto-5'nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleoside (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine-monophosphate, therefore driving the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promotor leads to upregulation of NT5E during hypoxia (3). NT5E biological roles span from lymphocyte adhesion (4, 5), to fibrosis (6), to regulation of nociception (7, 8).
Product Categories/Family for anti-NT5E antibody
References
1. Le Hir, M. and Kaissling, B. (1993) Am J Physiol 264, F377-87. 2. Schulte, G. and Fredholm, B.B. (2003) Cell Signal 15, 813-27. 3. Synnestvedt, K. et al. (2002) J Clin Invest 110, 993-1002. 4. Arvilommi, A.M. et al. (1997) Eur J Immunol 27, 248-54. 5. Airas, L. and Jalkanen, S. (1996) Blood 88, 1755-64. 6. Peng, Z. et al. (2008) Nucleosides Nucleotides Nucleic Acids 27, 821-4. 7. Sowa, N.A. et al. (2010) J Neurosci 30, 2235-44. 8. Zylka, M.J. (2011) Trends Mol Med 17, 188-96.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63kDa
NCBI Official Full Name
5'-nucleotidase isoform 2 preproprotein
NCBI Official Synonym Full Names
5'-nucleotidase ecto
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
NCBI Protein Information
5'-nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE; 5'-NT
NCBI Description
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Uniprot Description
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Research Articles on NT5E
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Product Notes
The NT5E nt5e (Catalog #AAA8500840) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NT5E Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NT5E can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NT5E nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NT5E, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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