5'-nucleotidase (NT5E) Recombinant Protein | NT5E recombinant protein

Recombinant Human 5'-nucleotidase (NT5E), partial

Cat. Num. AAA956512

• Gene Names: NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: 5'-nucleotidase (NT5E); Recombinant Human 5'-nucleotidase (NT5E); partial; Ecto-5'-nucleotidase; CD73; NT5E recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 28-547aa; Partial
• Sequence: ELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

SDS-PAGE

Related Product Information for NT5E recombinant protein

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Product Categories/Family for NT5E recombinant protein

Epigenetics and Nuclear Signaling

References

Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form.Misumi Y., Ogata S., Ohkubo K., Hirose S., Ikehara Y.Eur. J. Biochem. 191:563-569(1990) Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y., Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.DNA Res. 12:117-126(2005) The DNA sequence and analysis of human chromosome 6.Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.Nature 425:805-811(2003) Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73) -encoding gene.Hansen K.R., Resta R., Webb C.F., Thompson L.F.Gene 167:307-312(1995) Zanoni L., Rosi F., Pagani R., Marinello E. Characterization of soluble vs membrane-bound human placental 5'-nucleotidase.Klemens M.R., Sherman W.R., Holmberg N.J., Ruedi J.M., Low M.G., Thompson L.F.Biochem. Biophys. Res. Commun. 172:1371-1377(1990) Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.J. Proteome Res. 8:651-661(2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.Nat. Biotechnol. 27:378-386(2009) The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism.Garavaglia S., Bruzzone S., Cassani C., Canella L., Allegrone G., Sturla L., Mannino E., Millo E., De Flora A., Rizzi M.Biochem. J. 441:131-141(2012) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014) Crystal structure of the human ecto-5'-nucleotidase (CD73) insights into the regulation of purinergic signaling.Knapp K., Zebisch M., Pippel J., El-Tayeb A., Muller C.E., Strater N.Structure 20:2161-2173(2012) NT5E mutations and arterial calcifications.St Hilaire C., Ziegler S.G., Markello T.C., Brusco A., Groden C., Gill F., Carlson-Donohoe H., Lederman R.J., Chen M.Y., Yang D., Siegenthaler M.P., Arduino C., Mancini C., Freudenthal B., Stanescu H.C., Zdebik A.A., Chaganti R.K., Nussbaum R.L., Kleta R., Gahl W.A., Boehm M.N. Engl. J. Med. 364:432-442(2011) NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein.Fausther M., Lavoie E.G., Goree J.R., Baldini G., Dranoff J.A.PLoS ONE 9:E98568-E98568(2014)

NCBI and Uniprot Product Information

• NCBI GI #: 325651886
• NCBI GeneID: 4907
• NCBI Accession #: NP_001191742.1
• NCBI GenBank Nucleotide #: NM_001204813.1
• UniProt Accession #: P21589; O75520; Q5W116; B3KQI8
• Molecular Weight: 85kD
• NCBI Official Full Name: 5'-nucleotidase isoform 2 preproprotein
• NCBI Official Synonym Full Names: 5'-nucleotidase ecto
• NCBI Official Symbol: NT5E
• NCBI Official Synonym Symbols: NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• NCBI Protein Information: 5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: NT5E
• UniProt Synonym Gene Names: NT5; NTE; 5'-NT
• UniProt Entry Name: 5NTD_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.5; Membrane protein, GPI anchor; Membrane protein, integral; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; cytoplasm; membrane; plasma membrane

Molecular Function: 5'-nucleotidase activity

Biological Process: DNA metabolic process; leukocyte adhesion; purine nucleotide catabolic process; pyrimidine nucleoside catabolic process

Disease: Calcification Of Joints And Arteries