Human CD73/NT5E PicoKine ELISA Kit | NT5E elisa kit

Human CD73/NT5E PicoKine ELISA Kit

Cat. Num. AAA177581

• Gene Names: NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• Reactivity: Human
• Synonyms: CD73/NT5E PicoKine; Human CD73/NT5E PicoKine ELISA Kit; 5'-nucleotidase; 5' NT; 5' nucleotidase (CD73); 5' nucleotidase precursor; 5' nucleotidase; ecto; ecto (CD73); 5'-NT; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase ; NT5E elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human NT5E
• Sequence Length: 524

• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NT5E elisa kit

Principle of the Assay: The Picokine™ Human NT5E Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human NT5E with a 96-well strip plate that is pre-coated with antibody specific for NT5E. The detection antibody is a biotinylated antibody specific for NT5E. The capture antibody is polyclonal antibody from sheep, the detection antibody is polyclonal antibody from sheep. The kit contains recombinant Human NT5E with immunogen: Expression system for standard: CHO; Immunogen sequence: W27-K547. The kit is analytically validated with ready to use reagents. To measure Human NT5E, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human NT5E in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human NT5E in the sample.

Background/Introduction: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

NCBI and Uniprot Product Information

• NCBI GI #: 325651886
• NCBI GeneID: 4907
• NCBI Accession #: NP_001191742.1
• NCBI GenBank Nucleotide #: NM_001204813.1
• UniProt Accession #: P21589; O75520; Q5W116; B3KQI8
• Molecular Weight: 57,949 Da
• NCBI Official Full Name: 5'-nucleotidase isoform 2 preproprotein
• NCBI Official Synonym Full Names: 5'-nucleotidase ecto
• NCBI Official Symbol: NT5E
• NCBI Official Synonym Symbols: NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• NCBI Protein Information: 5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: NT5E
• UniProt Synonym Gene Names: NT5; NTE; 5'-NT
• UniProt Entry Name: 5NTD_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Membrane protein, GPI anchor; Membrane protein, integral; Phosphatase (non-protein); Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; cytoplasm; membrane; plasma membrane

Molecular Function: 5'-nucleotidase activity; ferrous iron binding; nucleotide binding

Biological Process: adenosine biosynthetic process; AMP catabolic process; brain development; dephosphorylation; DNA metabolic process; leukocyte adhesion; negative regulation of inflammatory response; positive regulation of lipid biosynthetic process; purine nucleotide biosynthetic process; purine nucleotide catabolic process; pyrimidine nucleoside catabolic process; response to aluminum ion

Disease: Calcification Of Joints And Arteries