Rabbit GSK3a (pS21) Antibody | anti-GSK3a (pS21) antibody

Rabbit anti Phospho- GSK-3alpha (pSer 21)

Cat. Num. AAA462105

• Gene Names: GSK3A; DKFZp686D0638
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Synonyms: GSK3a (pS21); Rabbit anti Phospho- GSK-3alpha (pSer 21); Rabbit anti-Phospho-GSK3á (pS21); Glycogen synthase kinase 3 alpha (GSK3á); anti-GSK3a (pS21) antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Specificity: This antibody recognizes GSK-3 alpha (pS21) with a phosphorylated site at Serine 9. It does not cross-react with non-phosphospecific peptide.
• Purity/Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-GSK3a (pS21) antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml
• Antigen Preparation: A synthetic peptide surrounding to the epitope -RTSSFA- with a phosphorylation site at Ser21 of human GSK3-alpha protein. This sequence is identical among human, mouse and rat.
• Positive Control: 3T3 cell lysate
• Cellular Location: Cytoplasmic
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-GSK3a (pS21) antibody

Glycogen Synthase Kinase-3 has two isoforms, GSK-3alpha and GSK3beta. GSK3 is a serine/threonine kinase that affects glycogen metabolism by phosphorylating and down-regulating the activity of muscle glycogen synthase as well as the regulation in Wnt signal pathway. GSK-3 alpha reduces the substrates beta-catenin and tau to imply an important role in Wnt signaling and tauopathies such as Alzheimer's disease..

References

Ayodeji A Ssuni, et al. GSK3alpha exhibits beta-catenin and tau directed kinase activities that are modulated by Wnt (2006). Eur J Neurosci. 24 (12): 3387-92. Yuko Kawakami, et al. Protein Kinase C _II Regulates Akt Phosphorylation on Ser-473 in a Cell Type- and Stimulus-specific Fashion (2004) THE J. Biol. Chem. 279 (46): 47720-47725.

NCBI and Uniprot Product Information

• NCBI GI #: 49574532
• NCBI GeneID: 2931
• NCBI Accession #: NP_063937.2
• NCBI GenBank Nucleotide #: NM_019884.2
• UniProt Accession #: P49840; O14959; Q68D16
• Molecular Weight: 52 kDa
• NCBI Official Full Name: glycogen synthase kinase-3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: GSK3A
• NCBI Official Synonym Symbols: DKFZp686D0638
• NCBI Protein Information: glycogen synthase kinase-3 alpha; GSK-3 alpha; OTTHUMP00000198366; OTTHUMP00000198367; OTTHUMP00000198368
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• UniProt Gene Name: GSK3A
• UniProt Entry Name: GSK3A_HUMAN

NCBI Description

Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM]

Uniprot Description

Function: Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN

Catalytic activity: ATP + [tau protein] = ADP + [tau protein] phosphate.

Subunit structure: Monomer. Interacts with ARRB2

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.Contains 1 protein kinase domain.