Mouse anti-Human Glycogen Synthase Kinase 3 alpha Monoclonal Antibody | anti-GSK3A antibody

Glycogen Synthase Kinase 3 alpha, phosphorylated (Ser21) (GSK3a)

Cat. Num. AAA631967

• Gene Names: GSK3A; DKFZp686D0638
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Glycogen Synthase Kinase 3 alpha; Monoclonal Antibody; phosphorylated (Ser21) (GSK3a); Anti -Glycogen Synthase Kinase 3 alpha; anti-GSK3A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3i16
• Specificity: Detects endogenous levels of GSK-3 alpha only when phosphorylated at Ser21. Species Crossreactivity: Human, mouse, rat, monkey and zebra fish.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, less than 0.02% sodium azide.

• Applicable Applications for anti-GSK3A antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:2000
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser21 of human GSK-3alpha.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from control and serum treated NIH/3T3 cells, using Phospho-GSK-3alpha (Ser21) (top), Phospho-GSK-3alpha/beta (Ser21/9) (middle) and GSK-3beta (bottom).)

Related Product Information for anti-GSK3A antibody

Glycogen synthase kinase 3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3alpha and Ser9 of GSK-3beta (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

Product Categories/Family for anti-GSK3A antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 49574532
• NCBI GeneID: 2931
• NCBI Accession #: NP_063937.2
• NCBI GenBank Nucleotide #: NM_019884.2
• UniProt Accession #: P49840; O14959; Q68D16
• Molecular Weight: 50,981 Da
• NCBI Official Full Name: glycogen synthase kinase-3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: GSK3A
• NCBI Official Synonym Symbols: DKFZp686D0638
• NCBI Protein Information: glycogen synthase kinase-3 alpha; GSK-3 alpha; OTTHUMP00000198366; OTTHUMP00000198367; OTTHUMP00000198368
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• Protein Family: Glycogen synthase kinase
• UniProt Gene Name: GSK3A
• UniProt Entry Name: GSK3A_HUMAN

NCBI Description

Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM]

Uniprot Description

GSK3A: a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized -catenin mutants that lack GSK3 phosphorylation sites. Inhibitor: AR-A014418

Protein type: EC 2.7.11.26; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: beta-catenin destruction complex; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; tau-protein kinase activity; ATP binding

Biological Process: glycogen metabolic process; proteasomal ubiquitin-dependent protein catabolic process; genetic imprinting; nerve growth factor receptor signaling pathway; negative regulation of insulin receptor signaling pathway; protein amino acid phosphorylation; negative regulation of transferase activity; positive regulation of cAMP biosynthetic process; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; nervous system development; negative regulation of TOR signaling pathway; cell migration; phosphoinositide-mediated signaling; Wnt receptor signaling pathway; fibroblast growth factor receptor signaling pathway; unfolded protein response; positive regulation of peptidyl-serine phosphorylation; negative regulation of glucose import; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cellular response to insulin stimulus; regulation of systemic arterial blood pressure; insulin receptor signaling pathway; positive regulation of protein catabolic process; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of heart contraction