Adenylate Cyclase 8 Recombinant Protein | ADCY8 recombinant protein

Recombinant Adenylate Cyclase 8, Brain (ADCY8)

Cat. Num. AAA2031505

• Gene Names: Adcy8; AC8; AW060868
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Adenylate Cyclase 8; Recombinant Adenylate Cyclase 8; Brain (ADCY8); ADCY8 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; KDTTSHT YLQYSGVVTW VAMTTQILAA GLGYGLLGDG IGYVLFTLFA TYSMLPLPLT WAILAGLGTS LLQVILQVVI PRLAVISINQ VVAQAVLFMC MNTAGIFISY LSDRAQRQAFLETRRCVEAR LRLETENQRQ E
• Sequence Length: 1249

• Applicable Applications for ADCY8 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 45.3kDa
• Accurate Molecular Mass (KD): 48kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, GST-tag, S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Lys234~Glu371
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 162319562
• NCBI GeneID: 11514
• Molecular Weight: 140,095 Da
• NCBI Official Full Name: Adenylate cyclase 8
• NCBI Official Synonym Full Names: adenylate cyclase 8
• NCBI Official Symbol: Adcy8
• NCBI Official Synonym Symbols: AC8; AW060868
• NCBI Protein Information: adenylate cyclase type 8
• UniProt Protein Name: Adenylate cyclase type 8
• Protein Family: Adenylate cyclase
• UniProt Gene Name: Adcy8
• UniProt Entry Name: ADCY8_MOUSE

Uniprot Description

ADCY8: This is a membrane-bound, calcium-stimulable adenylyl cyclase. May be involved in learning, in memory and in drug dependence. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Protein type: Transporter, aquaporin family; Adenylyl cyclase; Membrane protein, integral; EC 4.6.1.1; Nucleotide Metabolism - purine; Transporter; Lyase; Membrane protein, multi-pass

Cellular Component: membrane; plasma membrane; integral to membrane; intracellular

Molecular Function: calmodulin binding; protein C-terminus binding; phosphorus-oxygen lyase activity; metal ion binding; nucleotide binding; lyase activity; protein N-terminus binding; calcium- and calmodulin-responsive adenylate cyclase activity; ATP binding; adenylate cyclase activity

Biological Process: cAMP-mediated signaling; cyclic nucleotide biosynthetic process; long-term memory; cAMP biosynthetic process; G-protein signaling, adenylate cyclase activating pathway