Fatty Acid Synthase Recombinant Protein | FASN recombinant protein

Recombinant Fatty Acid Synthase (FASN)

Cat. Num. AAA2030247

• Gene Names: FASN; FAS; OA-519; SDR27X1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Fatty Acid Synthase; Recombinant Fatty Acid Synthase (FASN); FASN recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.6 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- PIEGSITV FHSLAAKLSV PTYGLQCTQA APLDSIPNLA AYYIDCIKQV QPEGPYRVAG YSFGACVAFE MCSQLQAQQG PAPAHNNLFL FDGSHTYVLA YTQSYRAKLT PGCEAEAEAE AICFFIKQFV DAEHSKVLEA LLPLKSLEDR VAAAVDLITR SHQSLDRRDL SFAAVSFYYK LRAADQYKPK AKYHGNVILL RAKTGGTYGE DLGADYNLSQ VCDGKVSVHI IEGDHRTLLE GRGLESIINI IHSSLAEPRVSVREG
• Sequence Length: 2511

• Applicable Applications for FASN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 32.3kDa
• Accurate Molecular Mass (KD): 30kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Pro2243~Gly2505
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 41872631
• NCBI GeneID: 2194
• NCBI Accession #: NP_004095.4
• NCBI GenBank Nucleotide #: NM_004104.4
• Molecular Weight: 273,427 Da
• NCBI Official Full Name: fatty acid synthase
• NCBI Official Synonym Full Names: fatty acid synthase
• NCBI Official Symbol: FASN
• NCBI Official Synonym Symbols: FAS; OA-519; SDR27X1
• NCBI Protein Information: fatty acid synthase
• UniProt Protein Name: Fatty acid synthase
• Protein Family: Fatty acid synthase
• UniProt Gene Name: FASN
• UniProt Synonym Gene Names: FAS
• UniProt Entry Name: FAS_HUMAN

NCBI Description

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

Uniprot Description

FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Protein type: EC 2.3.1.38; EC 2.3.1.39; Hydrolase; Acetyltransferase; EC 1.3.1.39; EC 2.3.1.41; Oxidoreductase; EC 1.1.1.100; EC 4.2.1.59; EC 2.3.1.85; EC 3.1.2.14; Lipid Metabolism - fatty acid biosynthesis; Lyase

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: Golgi apparatus; membrane; glycogen granule; mitochondrion; cytoplasm; melanosome; plasma membrane; cytosol

Molecular Function: protein homodimerization activity; fatty-acid synthase activity; myristoyl-[acyl-carrier-protein] hydrolase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; zinc ion binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; [acyl-carrier-protein] S-acetyltransferase activity; drug binding; 3-oxoacyl-[acyl-carrier-protein] reductase activity; protein binding; 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; [acyl-carrier-protein] S-malonyltransferase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity

Biological Process: osteoblast differentiation; vitamin metabolic process; acetyl-CoA metabolic process; pantothenate metabolic process; energy reserve metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid metabolic process; fatty acid biosynthetic process; water-soluble vitamin metabolic process