Rabbit GSK-3beta Polyclonal Antibody | anti-GSK-3beta antibody

GSK-3beta polyclonal antibody

Cat. Num. AAA565724

• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Protein A affinity purified.
• Synonyms: GSK-3beta; Polyclonal Antibody; GSK-3beta polyclonal antibody; Glycogen synthase kinase-3beta; pAb; anti-GSK-3beta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Polyclonal
• Specificity: Recognizes human, mouse, rat and bovine GSK-3beta. Detects a band of ~47kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 420

• Applicable Applications for anti-GSK-3beta antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: Western Blot (1:1'000, colorimetric).; Detects a band of ~47kDa by Western blot.
• Immunogen: Synthetic peptide corresponding to a portion of rat GSK-3beta. The sequence is completely conserved in human.
• Preparation and Storage: Long Term: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of human HeLa (heat shocked) cell lysate, probed with GSK3-beta pAb)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of human skeletal muscle tissue stained with GSK-3beta, pAb at 10µg/ml.)

Related Product Information for anti-GSK-3beta antibody

Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Product Categories/Family for anti-GSK-3beta antibody

Posttranslational Modifications; Cell Signaling; Proteolysis & Protein Degradation; Cell Death; Stem Cells; Cell & Tissue Markers

NCBI and Uniprot Product Information

• NCBI GI #: 125374
• NCBI GeneID: 84027
• UniProt Accession #: P18266
• Molecular Weight: 46,742 Da
• NCBI Official Full Name: Glycogen synthase kinase-3 beta
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: Gsk3b
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: Gsk3b
• UniProt Synonym Gene Names: GSK-3 beta; FA

NCBI Description

mediates Par6-atypical protein kinase C (aPKC) complex regulation of cell polarity; may induce apoptosis [RGD, Feb 2006]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: CMGC group; EC 2.7.11.1; EC 2.7.11.26; GSK family; GSK subfamily; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: axon; beta-catenin destruction complex; cell soma; centrosome; cytoplasm; cytosol; dendrite; dendritic shaft; dendritic spine; growth cone; lipid raft; membrane; microtubule; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic density; protein complex; ribonucleoprotein complex

Molecular Function: ATP binding; beta-catenin binding; integrin binding; ionotropic glutamate receptor binding; kinase activity; NF-kappaB binding; p53 binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau protein binding; tau-protein kinase activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: aging; axonogenesis; cell migration; circadian rhythm; epithelial to mesenchymal transition; ER overload response; establishment and/or maintenance of cell polarity; establishment of cell polarity; fat cell differentiation; genetic imprinting; glycogen metabolic process; hippocampus development; myoblast fusion; myotube differentiation; negative regulation of apoptosis; negative regulation of dendrite morphogenesis; negative regulation of MAP kinase activity; negative regulation of neuron maturation; negative regulation of NFAT protein import into nucleus; negative regulation of nitric-oxide synthase activity; negative regulation of protein binding; negative regulation of protein complex assembly; organ morphogenesis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of apoptosis; positive regulation of axon extension; positive regulation of cell-matrix adhesion; positive regulation of GTPase activity; positive regulation of neuron apoptosis; positive regulation of peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of protein complex assembly; positive regulation of protein export from nucleus; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein export from nucleus; re-entry into mitotic cell cycle; regulation of axon extension; regulation of axonogenesis; regulation of dendrite morphogenesis; regulation of microtubule-based process; regulation of neuronal synaptic plasticity; response to activity; response to drug; response to estradiol stimulus; response to insulin stimulus; response to lithium ion; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin