Western Blot (WB)
(Figure 1. Western blot analysis of Cdc25c using anti-Cdc25c antibody (MBS1753429).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat pancreas tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Cdc25c antigen affinity purified polyclonal antibody (Catalog # MBS1753429) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Cdc25c at approximately 53KD. The expected band size for Cdc25c is at 53KD. )
Rabbit anti-Mouse, Rat Cdc25c Polyclonal Antibody | anti-Cdc25c antibody
Anti-Cdc25c Antibody
Gene Names
Cdc25c; Cdc25
Reactivity
Mouse, Rat
Applications
Western Blot, ELISA
Purity
Immunogen affinity purified.
Synonyms
Cdc25c; Polyclonal Antibody; Anti-Cdc25c Antibody; Cdc25m1; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; cell division cycle 25C; anti-Cdc25c antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for Cdc25c detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Applicable Applications for anti-Cdc25c antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml|Rat|
Direct ELISA: 0.1-0.5ug/ml|Mouse|
Direct ELISA: 0.1-0.5ug/ml|Mouse|
Immunogen
E Coli-derived mouse Cdc25c recombinant protein (Position: K24-Q447).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of Cdc25c using anti-Cdc25c antibody (MBS1753429).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat pancreas tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Cdc25c antigen affinity purified polyclonal antibody (Catalog # MBS1753429) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Cdc25c at approximately 53KD. The expected band size for Cdc25c is at 53KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Cdc25c using anti-Cdc25c antibody (MBS1753429).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat pancreas tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Cdc25c antigen affinity purified polyclonal antibody (Catalog # MBS1753429) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Cdc25c at approximately 53KD. The expected band size for Cdc25c is at 53KD. )
Related Product Information for anti-Cdc25c antibody
M-phase inducer phosphatase 3 is an enzyme that in humans is encoded by the CDC25C gene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
References
1. "Entrez Gene: CDC25C cell division cycle 25 homolog C (S. pombe)".
2. Gould KL, Moreno S, Tonks NK, Nurse P (Feb 1991). "Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase". Science 250 (4987): 1573-6.
3. Sartor H, Ehlert F, Grzeschik KH, et al. (1992). "Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively". Genomics 13(3): 911-2.
2. Gould KL, Moreno S, Tonks NK, Nurse P (Feb 1991). "Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase". Science 250 (4987): 1573-6.
3. Sartor H, Ehlert F, Grzeschik KH, et al. (1992). "Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively". Genomics 13(3): 911-2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,046 Da
NCBI Official Full Name
M-phase inducer phosphatase 3
NCBI Official Synonym Full Names
cell division cycle 25C
NCBI Official Symbol
Cdc25c
NCBI Official Synonym Symbols
Cdc25
NCBI Protein Information
M-phase inducer phosphatase 3; cell cycle phosphatase CDC25C; dual specificity phosphatase Cdc25C; cell division cycle control protein 25C
UniProt Protein Name
M-phase inducer phosphatase 3
UniProt Gene Name
Cdc25c
UniProt Synonym Gene Names
Cdc25m1
UniProt Entry Name
MPIP3_MOUSE
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Product Notes
The Cdc25c cdc25c (Catalog #AAA1753429) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cdc25c Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cdc25c can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Rat| Direct ELISA: 0.1-0.5ug/ml|Mouse|. Researchers should empirically determine the suitability of the Cdc25c cdc25c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdc25c, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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