Testing Data
Mouse GSK-3a/b (Tyr-279/Tyr-216) Monoclonal Antibody
GSK-3alpha/beta (Tyr-279/Tyr-216), phosphospecific
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot
Purity
Protein A Purified
Synonyms
GSK-3a/b (Tyr-279/Tyr-216); Monoclonal Antibody; GSK-3alpha/beta (Tyr-279/Tyr-216); phosphospecific; phospho-specific; Glycogen synthase kinase-3 beta; anti-GSK-3a/b (Tyr-279/Tyr-216) antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
This antibody detects 46/50 kDa* proteins corresponding to the apparent molecular mass of GSK-3b and GSK-3a on SDS-PAGE immunoblots of pervanadate treated rabbit fibroblasts, as well as treated human SKN-SH and A431 cells.
Purity/Purification
Protein A Purified
Form/Format
Mouse monoclonal purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-GSK-3a/b (Tyr-279/Tyr-216) antibody
ELISA, Western Blot
Application Notes
ELISA: 1:2000
Western Blot: 1:1000
Western Blot: 1:1000
Immunogen
Clone M132 was generated from a phospho-GSK-3b (Tyr-216) synthetic peptide (coupled to KLH) corresponding to amino acid residues around
tyrosine 216 of human GSK-3b. This peptide sequence is also found in GSK-3a (Tyr-279) and is highly conserved in GSK-3 genes in rat and mouse.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for anti-GSK-3a/b (Tyr-279/Tyr-216) antibody
Glycogen synthase kinase-3 (GSK-3) has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two GSK-3 genes (alpha and beta) have been cloned in mammals and these kinase homologues show strong sequence conservation within their catalytic domain. GSK-3beta plays a critical role in cell survival by phosphorylating nuclear factor-kappa-B (NF- kappa-B) p65 subunit, leading to NF-kappa-B transactivation in hepatocytes. Phosphorylation regulates the activity of both GSK-3 genes. MEK1/2 can phosphorylate tyrosine 216 (tyrosine 279 in GSK-3 alpha), which stimulates GSK-3 kinase activity. Tyr-216 phosphorylation is required for GSK-mediated down-regulation of beta-catenin activity. Also, TRAIL stimulation can increase Tyr-216 phosphorylation, and GSK-3beta activity may suppress TRAIL-induced apoptosis. Inactiviation of GSK-3 occurs through Akt phosphorylation of serine 9 of GSK-3beta (Serine 21 in GSK-3alpha). This phosphorylation may be involved in later phases of neuronal apoptosis.
References
• Hagen, T. et al. (2002) J Biol Chem. 277(26):23330.
• Takahashi-Yanaga, F. et al. (2004) Biochem Biophys Res Commun. 316(2):411.
• Xinbo, L. et al. (2003) Mol Cancer Ther. 2:1215.
• Ding, Y. et al. (2008) J Neurosci. 28(45):11622. (WB: Mouse cortex, hippocampus)
• Takahashi-Yanaga, F. et al. (2004) Biochem Biophys Res Commun. 316(2):411.
• Xinbo, L. et al. (2003) Mol Cancer Ther. 2:1215.
• Ding, Y. et al. (2008) J Neurosci. 28(45):11622. (WB: Mouse cortex, hippocampus)
Similar Products
Product Notes
The GSK-3a/b (Tyr-279/Tyr-216) (Catalog #AAA470015) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GSK-3alpha/beta (Tyr-279/Tyr-216), phosphospecific reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GSK-3a/b (Tyr-279/Tyr-216) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. ELISA: 1:2000 Western Blot: 1:1000. Researchers should empirically determine the suitability of the GSK-3a/b (Tyr-279/Tyr-216) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK-3a/b (Tyr-279/Tyr-216), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
