Rabbit GSK-3Beta Polyclonal Antibody | anti-GSK3B antibody

GSK-3Beta Antibody: RPE

Cat. Num. AAA805125

• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Western Blot, Immunohistochemistry
• Synonyms: GSK-3Beta; Polyclonal Antibody; GSK-3Beta Antibody: RPE; Glycogen Synthase kinase 3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK3B; GSK3B_Human; GSK3beta isoform; anti-GSK3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Polyclonal
• Form/Format: Affinity Purified
• Concentration: 1.3mg/mL (varies by lot)
• Sequence Length: 420

• Applicable Applications for anti-GSK3B antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: 1:2000 (WB)
• Conjugate: RPE
• Storage Buffer: PBS pH7.4
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Human Cell line lysates showing detection of GSK3 Beta protein using Rabbit Anti-GSK3 Beta Polyclonal Antibody (SPC-169). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-GSK3 Beta Polyclonal Antibody (SPC-169) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Related Product Information for anti-GSK3B antibody

Background Info: Detects ~47kDa.

Scientific Background: Glycogen synthase kinase 3, is a serine/threonine protein kinase that mediates the addition of phosphate molecules onto serine and threonine amino acid residues.  It has also been shown to regulate immune and migratory processes, and is tied to pathways of cell proliferation and apoptosis (1-3). In mammals, it is encoded by two genes- GSK-3Alpha and GSK-3Beta. They are structurally similar, but functionally non-identical. GSK-3Alpha is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3Beta negatively regulates cardiac hypertrophy and cardiac development through its effect on WNT signaling (4). GSK-3 alpha and GSK-3 beta share 85% amino acid identity. GSK-3 has been implicated in a number of diseases including Type II diabetes, Alzheimer's, cancer and bipolar (4, 5).

Product Categories/Family for anti-GSK3B antibody

Cell Signaling

References

1. Jope R.S. (2007) Neurochem Res. 32(4-5): 577-595. 2. Wang H. (2011) Cytokind. 53(2): 130-140. 3. Mills C.N. (2011) Front Mol Neurosci. 47(4): 47. 4. Hardt S.E., Sadoshima J. (2002) Circ Res. 90(10): 1055-1063. 5. Doble D.W., Woodgett J.R. (2003) J Cell Sci. 116(Pt7): 1175-1186.

NCBI and Uniprot Product Information

• NCBI GI #: 225903437
• NCBI GeneID: 2932
• NCBI Accession #: NP_001139628.1
• NCBI GenBank Nucleotide #: NM_001146156.1
• UniProt Accession #: P49841; Q9BWH3; Q9UL47; D3DN89
• Molecular Weight: 48,034 Da
• NCBI Official Full Name: glycogen synthase kinase-3 beta isoform 2
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; serine/threonine-protein kinase GSK3B
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• Protein Family: Glycogen synthase kinase
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta
• UniProt Entry Name: GSK3B_HUMAN

NCBI Description

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: Kinase, protein; EC 2.7.11.1; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 3q13.3

Cellular Component: nucleoplasm; centrosome; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic spine; dendritic shaft; beta-catenin destruction complex; cytosol; nucleus; ribonucleoprotein complex; lipid raft

Molecular Function: p53 binding; beta-catenin binding; protein kinase binding; integrin binding; protein serine/threonine kinase activity; NF-kappaB binding; ionotropic glutamate receptor binding; protein binding; ubiquitin protein ligase binding; tau-protein kinase activity; kinase activity; tau protein binding; ATP binding

Biological Process: circadian rhythm; glycogen metabolic process; fat cell differentiation; axon guidance; negative regulation of MAP kinase activity; re-entry into mitotic cell cycle; genetic imprinting; positive regulation of protein binding; nerve growth factor receptor signaling pathway; positive regulation of axon extension; protein amino acid autophosphorylation; response to lithium ion; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; negative regulation of protein binding; positive regulation of cell-matrix adhesion; ER overload response; myoblast fusion; negative regulation of glycogen biosynthetic process; response to drug; epidermal growth factor receptor signaling pathway; regulation of microtubule-based process; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; organ morphogenesis; peptidyl-serine phosphorylation; negative regulation of neuron maturation; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; epithelial to mesenchymal transition; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; regulation of neuronal synaptic plasticity; negative regulation of apoptosis