Standard Curve (Sample)
(Human CHI3L1 PicoKine ELISA Kit standard curve)
Human Chitinase 3-Like 1/YKL-40 ELISA Kit | CHI3L1 elisa kit
Human Chitinase 3-Like 1/YKL-40 PicoKine Quick ELISA Kit
Gene Names
CHI3L1; GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
Reactivity
Human
Applications
ELISA
Synonyms
Chitinase 3-Like 1/YKL-40; N/A; Human Chitinase 3-Like 1/YKL-40 PicoKine Quick ELISA Kit; CHI3L1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CHI3L1
Assay Type
Sandwich
Samples
Cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<10 pg/ml
Applicable Applications for CHI3L1 elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Human CHI3L1 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human CHI3L1 PicoKine ELISA Kit standard curve)
Related Product Information for CHI3L1 elisa kit
Background: Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
Principle of the Assay: The Quick Picokine™ Human CHI3L1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CHI3L1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human CHI3L1 protein as standards. To measure Human CHI3L1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CHI3L1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CHI3L1 in the sample.
Principle of the Assay: The Quick Picokine™ Human CHI3L1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CHI3L1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human CHI3L1 protein as standards. To measure Human CHI3L1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CHI3L1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CHI3L1 in the sample.
Product Categories/Family for CHI3L1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,625 Da
NCBI Official Full Name
chitinase-3-like protein 1
NCBI Official Synonym Full Names
chitinase 3-like 1 (cartilage glycoprotein-39)
NCBI Official Symbol
CHI3L1
NCBI Official Synonym Symbols
GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
NCBI Protein Information
chitinase-3-like protein 1; 39 kDa synovial protein; cartilage glycoprotein 39
UniProt Protein Name
Chitinase-3-like protein 1
UniProt Gene Name
CHI3L1
UniProt Synonym Gene Names
CGP-39; GP-39; hCGP-39
UniProt Entry Name
CH3L1_HUMAN
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Product Notes
The Human CHI3L1 chi3l1 (Catalog #AAA126822) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126822 ELISA Kit recognizes Human CHI3L1. AAA Biotech's Chitinase 3-Like 1/YKL-40 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CHI3L1 chi3l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chitinase 3-Like 1/YKL-40, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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