Typical Testing Data/Standard Curve (for reference only)
Mouse PaRathyroid Hormone Related Protein ELISA Kit | PTHrP elisa kit
Mouse PaRathyroid Hormone Related Protein ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PTHrP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PTHrP antibody and an PTHrP-HRP conjugate. The assay sample and buffer are incubated together with PTHrP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTHrP concentration since PTHrP from samples and PTHrP-HRP conjugate compete for the anti-PTHrP antibody binding site. Since the number of sites is limited, as more sites are occupied by PTHrP from the sample, fewer sites are left to bind PTHrP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTHrP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
induces vasodilation; may play a neuroprotective role in response to ischemic brain injury [RGD, Feb 2006]
Uniprot Description
PTHrP: a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Three alternatively spliced isoforms have been described.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; extracellular region; intracellular; nucleus
Molecular Function: peptide hormone receptor binding; hormone activity
Biological Process: negative regulation of transcription factor activity; negative regulation of chondrocyte differentiation; G-protein signaling, adenylate cyclase activating pathway; bone mineralization; osteoblast development; epithelial cell differentiation; regulation of gene expression; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; protein processing; regulation of chondrocyte differentiation; alveolus development; surfactant homeostasis; skeletal development; endochondral ossification; endoderm development
Research Articles on PTHrP
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Product Notes
The Mouse PTHrP pthlh (Catalog #AAA722308) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA722308 ELISA Kit recognizes Mouse PTHrP. It is sometimes possible for the material contained within the vial of "PaRathyroid Hormone Related Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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