Typical Testing Data/Standard Curve (for reference only)
Rabbit PaRathyroid Hormone Related Protein ELISA Kit | PTHrP elisa kit
Rabbit PaRathyroid Hormone Related Protein ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This PTHrP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit PTHrP. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY PTHrP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PTHrP. Standards or samples are then added to the microtiter plate wells and PTHrP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PTHrP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PTHrP are added to each well to “sandwich” the PTHrP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PTHrP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTHrP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
induces vasodilation; may play a neuroprotective role in response to ischemic brain injury [RGD, Feb 2006]
Uniprot Description
PTHrP: a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Three alternatively spliced isoforms have been described.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; extracellular region; intracellular; nucleus
Molecular Function: peptide hormone receptor binding; hormone activity
Biological Process: negative regulation of transcription factor activity; negative regulation of chondrocyte differentiation; G-protein signaling, adenylate cyclase activating pathway; bone mineralization; osteoblast development; epithelial cell differentiation; regulation of gene expression; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; protein processing; regulation of chondrocyte differentiation; surfactant homeostasis; skeletal development; alveolus development; endochondral ossification; endoderm development
Research Articles on PTHrP
Similar Products
Product Notes
The Rabbit PTHrP pthlh (Catalog #AAA720859) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720859 ELISA Kit recognizes Rabbit PTHrP. It is sometimes possible for the material contained within the vial of "PaRathyroid Hormone Related Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
