Bovine Luteinizing Hormone ELISA Kit | LH elisa kit
Bovine Luteinizing Hormone ELISA Kit
Reactivity
Bovine
Synonyms
Luteinizing Hormone; Bovine Luteinizing Hormone ELISA Kit; LH elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of LH. No significant cross-reactivity or interference between LH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LH and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LH elisa kit
Intended Uses: This LH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine LH. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: LH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LH antibody and an LH-HRP conjugate. The assay sample and buffer are incubated together with LH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LH concentration since LH from samples and LH-HRP conjugate compete for the anti-LH antibody binding site. Since the number of sites is limited, as more sites are occupied by LH from the sample, fewer sites are left to bind LH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LH concentration in each sample is interpolated from this standard curve.
Principle of the Assay: LH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LH antibody and an LH-HRP conjugate. The assay sample and buffer are incubated together with LH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LH concentration since LH from samples and LH-HRP conjugate compete for the anti-LH antibody binding site. Since the number of sites is limited, as more sites are occupied by LH from the sample, fewer sites are left to bind LH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LH concentration in each sample is interpolated from this standard curve.
