Goat Parathyroid Hormone Related Protein ELISA Kit | PTHRP elisa kit

Goat Parathyroid Hormone Related Protein ELISA Kit

Cat. Num. AAA739761

• Gene Names: Pthlh; PLP
• Reactivity: Goat
• Synonyms: Parathyroid Hormone Related Protein; Goat Parathyroid Hormone Related Protein ELISA Kit; PTHRP elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This PTHRP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat PTHRP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTHRP elisa kit

Principle of the Assay: PTHRP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTHRP antibody and an PTHRP-HRP conjugate. The assay sample and buffer are incubated together with PTHRP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTHRP concentration since PTHRP from samples and PTHRP-HRP conjugate compete for the anti-PTHRP antibody binding site. Since the number of sites is limited, as more sites are occupied by PTHRP from the sample, fewer sites are left to bind PTHRP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTHRP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PTHRP elisa kit

Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 6981438
• NCBI GeneID: 24695
• NCBI Accession #: NP_036768.1
• NCBI GenBank Nucleotide #: NM_012636.1
• UniProt Accession #: P13085
• Molecular Weight: 20,204 Da
• NCBI Official Full Name: parathyroid hormone-related protein preproprotein
• NCBI Official Synonym Full Names: parathyroid hormone-like hormone
• NCBI Official Symbol: Pthlh
• NCBI Official Synonym Symbols: PLP
• NCBI Protein Information: parathyroid hormone-related protein; PTHrP; PTH-rP; parathyroid-like peptide; parathyroid hormone-like peptide; parathyroid hormone-like protein
• UniProt Protein Name: Parathyroid hormone-related protein
• UniProt Gene Name: Pthlh
• UniProt Synonym Gene Names: Pthrp; PTH-rP; PTHrP; PLP
• UniProt Entry Name: PTHR_RAT

NCBI Description

induces vasodilation; may play a neuroprotective role in response to ischemic brain injury [RGD, Feb 2006]

Uniprot Description

PTHrP: a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Three alternatively spliced isoforms have been described.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; extracellular region; intracellular; nucleus

Molecular Function: peptide hormone receptor binding; hormone activity

Biological Process: negative regulation of transcription factor activity; negative regulation of chondrocyte differentiation; G-protein signaling, adenylate cyclase activating pathway; bone mineralization; osteoblast development; epithelial cell differentiation; regulation of gene expression; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; protein processing; regulation of chondrocyte differentiation; alveolus development; surfactant homeostasis; skeletal development; endoderm development; endochondral ossification