HRP Conjugated Streptavidin Protein
HRP Conjugated Streptavidin
Applications
Dot Blot, Western Blot, ELISA
Synonyms
HRP Conjugated Streptavidin; HRP Conjugated Streptavidin protein
Specificity
This HRP conjugated streptavidin.
Form/Format
Concentrated, Liquid
Concentration
1mg/ml (varies by lot)
Applicable Applications for HRP Conjugated Streptavidin protein
Dot Blot, Western Blot (WB), ELISA (EIA)
Application Notes
Dot Blot: 0.25-0.5ug/mL (ECL Detection)
Western Blotting: 0.1-0.3ug/ml (ECL detection)
Western Blotting: 0.3-2ug/ml (DAB detection)
Direct ELISA: 0.025-0.05ug/ml (TMB detection).
Optimal working dilutions must be determined by end users.
Western Blotting: 0.1-0.3ug/ml (ECL detection)
Western Blotting: 0.3-2ug/ml (DAB detection)
Direct ELISA: 0.025-0.05ug/ml (TMB detection).
Optimal working dilutions must be determined by end users.
Contents
1 mg of peroxidase conjugated streptavidin; 0.01M PBS (pH 7.4), 50% glycerol
Labeling Method
Streptavidin is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK
Preparation of Dilutent Buffer
Use 0.01 M TBS or 0.01 M PBS to dilute:
Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g Na2HPO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g Na2HPO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Conjugation
Peroxidase
Preparation and Storage
Store at 4°C for one year.
Related Product Information for HRP Conjugated Streptavidin protein
Description: Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin. And lots of enzymes can ensure the streptavidin-HRP with high sensitivity.
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Product Notes
The HRP Conjugated Streptavidin (Catalog #AAA176633) is a Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HRP Conjugated Streptavidin can be used in a range of immunoassay formats including, but not limited to, Dot Blot, Western Blot (WB), ELISA (EIA). Dot Blot: 0.25-0.5ug/mL (ECL Detection) Western Blotting: 0.1-0.3ug/ml (ECL detection) Western Blotting: 0.3-2ug/ml (DAB detection) Direct ELISA: 0.025-0.05ug/ml (TMB detection). Optimal working dilutions must be determined by end users. Researchers should empirically determine the suitability of the HRP Conjugated Streptavidin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRP Conjugated Streptavidin, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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