Immunohistochemistry (IHC)
(CRM1 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using HRP Conjugated anti-Rabbit IgG SABC Kit.)
HRP Conjugated anti-Rabbit IgG Detection Kit
HRP Conjugated anti-Rabbit IgG SABC Kit
Applications
Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
Synonyms
HRP Conjugated anti-Rabbit IgG; HRP Conjugated anti-Rabbit IgG SABC Kit; HRP Conjugated anti-Rabbit IgG detection kit
Form/Format
Concentrated (Need dilution)
Applicable Applications for HRP Conjugated anti-Rabbit IgG detection kit
Immunohistochemistry (IHC) Paraffin, IImmunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC)
Application Notes
IHC(P): 1:50-200, IHC(F): 1:50-200, ICC: 1:50-200. Optimal dilutions should be determined by end users.
Preparation and Storage
4 degree C for one year. Avoid freezing
Immunohistochemistry (IHC)
(CRM1 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using HRP Conjugated anti-Rabbit IgG SABC Kit.)
Immunohistochemistry (IHC)
(CRM1 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using HRP Conjugated anti-Rabbit IgG SABC Kit.)
Immunohistochemistry (IHC)
(ZAP70 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- ZAP70 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using HRP Conjugated anti-Rabbit IgG SABC Kit.)
Immunohistochemistry (IHC)
(ZAP70 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- ZAP70 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using HRP Conjugated anti-Rabbit IgG SABC Kit.)
Related Product Information for HRP Conjugated anti-Rabbit IgG detection kit
Background/Introduction: SABC (StreptAvidin-Biotin Complex) is specially designed for displaying the distribution of antigens in tissues and cells in immunochemistry and other immunodetection analyses. Streptavidin is a 53,000 dalton protein purified from the bacterium Streptomyces avidinii. Streptavidin has extraordinarily strong affinity to biotin molecules. The dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L, a million times higher than the typical affinity between antigens and their antibodies. Streptavidin has very low non-specific binding to tissues and cells, due to its nearly neutral isoelectric point (IP=6.0~6.5). Therefore, immunohistochemical analyses based on streptavidin-biotin complex has extremely low background. Furthermore, this kit has high sensitivity because each complex it generates has a large number of peroxidase and streptavidin molecules. In brief, SABC offers high specificity, low background and ease-of-use.
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Product Notes
The HRP Conjugated anti-Rabbit IgG (Catalog #AAA176439) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HRP Conjugated anti-Rabbit IgG can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, IImmunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC). IHC(P): 1:50-200, IHC(F): 1:50-200, ICC: 1:50-200. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the HRP Conjugated anti-Rabbit IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRP Conjugated anti-Rabbit IgG, Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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