Testing Data
(PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 ug/mL. FITC Conjugated Protein A was used to detect the primary antibody at 30 ug/mL.)
FITC Conjugated Protein A Secondary Antibody
FITC Conjugated Protein A
Applications
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Synonyms
FITC Conjugated Protein A; FITC Conjugated Protein A secondary antibody
Form/Format
Concentrated, Liquid
Concentration
1mg/ml (varies by lot)
Applicable Applications for FITC Conjugated Protein A secondary antibody
Flowcytometry (FCM);Immunocytochemistry (ICC); Indirect Immunofluorescence (IF); Immunohistochemistry (IHC)
Application Notes
FCM: 1:100
ICC: 1:64-128
Indirect IF: 1:64-128
IHC (F): 1:64-128
Optimal working dilutions must be determined by end users.
ICC: 1:64-128
Indirect IF: 1:64-128
IHC (F): 1:64-128
Optimal working dilutions must be determined by end users.
Contents
0.5 mg of FITC conjugated protein A, F/P=3/1; 0.01 M PBS; 0.01 % Thimerosal,50% glycerol.
Preparation of Diluent Buffer
Add reagent grade BSA into 0.01 M PBS (PH7.2-7.6) or TBS buffer and make BSA at a concentration of 1 %. Use the above diluent buffer to dilute. See "Recommended Dilutions" below for details.
Preparation of 0.01 M TBS: Add 1.2g Tris, 8.5g NaCI; 450IJI of purified acetic acid or 700IJI of concentrated hydrochloric acid to 1000ml H20 and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1 L.
Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HP04 and 0.2g NaH2P04 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1 L.
Preparation of 0.01 M TBS: Add 1.2g Tris, 8.5g NaCI; 450IJI of purified acetic acid or 700IJI of concentrated hydrochloric acid to 1000ml H20 and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1 L.
Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HP04 and 0.2g NaH2P04 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1 L.
Labeling Method
Protein A is conjugated to FITC by means of a method described by Hijmans,w.,et al. (Reference: Hijmans,w.,et al. Clin.Exp.lmmunol.,4, 457(1969))
Preparation and Storage
At 4 degree C for one year.
Testing Data
(PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 ug/mL. FITC Conjugated Protein A was used to detect the primary antibody at 30 ug/mL.)
Testing Data
(PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 ug/mL. FITC Conjugated Protein A was used to detect the primary antibody at 30 ug/mL.)
Related Product Information for FITC Conjugated Protein A secondary antibody
Description: Protein A is the component of the cytoderm of staphylococcus aureus. It was regarded as a kind of broad spectrum secondary antibody, because it can bind Fc fragments of a majority of immunoglobulins. It can react with guinea pig, rabbit globulin and human IgG1, 2, 4 strongly and react with mouse, bovine globulin and human IgA,E,M weakly.
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Product Notes
The FITC Conjugated Protein A (Catalog #AAA176623) is a Secondary Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FITC Conjugated Protein A can be used in a range of immunoassay formats including, but not limited to, Flowcytometry (FCM);Immunocytochemistry (ICC); Indirect Immunofluorescence (IF); Immunohistochemistry (IHC). FCM: 1:100 ICC: 1:64-128 Indirect IF: 1:64-128 IHC (F): 1:64-128 Optimal working dilutions must be determined by end users. Researchers should empirically determine the suitability of the FITC Conjugated Protein A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FITC Conjugated Protein A, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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