10 nm Colloidal Gold Conjugated Protein A Secondary Antibody

10 nm Colloidal Gold Conjugated Protein A

Cat. Num. AAA176686

• Applications: Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
• Synonyms: 10 nm Colloidal Gold Conjugated Protein A; 10 nm Colloidal Gold Conjugated Protein A secondary antibody

• Form/Format: 0.1 mg of colloidal gold conjugated protein A, 0.01M PBS, 0.01% Thimerosal, 50% glycerol.

• Applicable Applications for 10 nm Colloidal Gold Conjugated Protein A secondary antibody: Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), EM
• Application Notes: Electron Microscopy - 1:20-50; Immunohistochemistry(Paraffin-embedded Section) - 1:50-100; Immunohistochemistry(Frozen Section) - 1:50-100; Immunocytochemistry - 1:50-100; ; Optimal working dilutionsmust be determined by end users.
• Preparation of Diluent Buffer: The conjugate should be diluted for most applications, using 0.01M PBS or TBS, containing 1% BSA.; - Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl, 450 ul of purified acetic acid or 700 ul of concentrated hydrochloric acid into 1000 ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.; - Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 into 1000ml distill
• Preparation and Storage: Upon receipt store 10 nm Colloidal Gold Conjugated Protein A at 4°C. 10 nm Colloidal Gold Conjugated Protein A

Testing Data

(Binding capabilities for immunoglobulin proteins and protein A, G and A/G)

Product Information Sheet

(Binding will only occur if the appropriate kappa light chains are present. The binding affinity only refers to species and subtypes with the correct kappa light chains. Lambda light chains and some kappa light chains will not bindNotes: W= weak bindingM=medium bindingS=strong bindingNB=no binding)

Related Product Information for 10 nm Colloidal Gold Conjugated Protein A secondary antibody

Protein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs (Table 1). It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis. Recombinant Protein A is produced in E.coli and essentially functions the same as native Protein A.

Protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, b-galactosidase, and alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including Western blotting, immunohistochemistry, and ELISA applications.

Protein A is conjugated to 10 nm colloidal gold. This gold conjugated protein A generates electron dense particles that are visible using electron microscopy.