Testing Data
(PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 ug/mL. FITC Conjugated Goat Anti-mouse IgG secondary antibody was used to detect the primary antibody at 30 ug/mL.)
FITC Conjugated Goat Anti-mouse IgG Secondary Antibody
FITC Conjugated Goat Anti-mouse IgG secondary antibody
Applications
Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Synonyms
FITC Conjugated Goat Anti-mouse IgG; Polyclonal Secondary Antibody; FITC Conjugated Goat Anti-mouse IgG secondary antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Specificity
This FITC conjugated antibody is specific for mouse IgG and shows no cross-reactivity with human, bovine, rabbit IgG.
Form/Format
Concentrated, Liquid
Concentration
1mg/ml (varies by lot)
Applicable Applications for FITC Conjugated Goat Anti-mouse IgG secondary antibody
Immunohistochemistry (IHC-P) Paraffin, Immunohistochemistry (IHC-F) Frozen, Immunocytochemistry (ICC), Flow Cytometry (FCM)
Application Notes
Flowcytometry: 5-10uL/106cells
Immunohistochemistry(Paraffin-embedded Section): 10-50ug/mL
Immunohistochemistry(Frozen Section): 10-50ug/mL
Immunocytochemistry: 10-50ug/mL
Optimal working dilutions must be determined by end users.
Immunohistochemistry(Paraffin-embedded Section): 10-50ug/mL
Immunohistochemistry(Frozen Section): 10-50ug/mL
Immunocytochemistry: 10-50ug/mL
Optimal working dilutions must be determined by end users.
Immunogen
Mouse IgG (whole molecular).
Contents
0.5 mg of FITC conjugated specific antibody (purity is above 99%), FITC: Ab = 4-6 : 1, 0.01M PBS, 0.01% Thimerosal, 50% glycerol. The emission and filtration wavelength of FITC are 495 nm and 525 nm respectively.
Labeling Method
Goat anti-mouse IgG is conjugated to FITC by means of a method described by Hijmans,W.,et al. (Reference: Hijmans,W.,et al. Clin.Exp.Immunol.,4, 457(1969))
Preparation of Diluent Buffer
Add reagent grade BSA into 0.01 M PBS (PH7.2-7.6) or TBS buffer and make BSA at a concentration of 1%. Use the above diluent buffer to dilute. See “Recommended Dilutions” below for details.
Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation and Storage
At 4 degree C for one year.
Testing Data
(PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 ug/mL. FITC Conjugated Goat Anti-mouse IgG secondary antibody was used to detect the primary antibody at 30 ug/mL.)
Testing Data
(PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody at 1 ug/mL. FITC Conjugated Goat Anti-mouse IgG secondary antibody was used to detect the primary antibody at 30 ug/mL.)
Related Product Information for FITC Conjugated Goat Anti-mouse IgG secondary antibody
Description: This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins and bovine serum proteins to ensure minimal cross reactivity in tissue or cell preparations.
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Product Notes
The FITC Conjugated Goat Anti-mouse IgG (Catalog #AAA176687) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FITC Conjugated Goat Anti-mouse IgG can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC-P) Paraffin, Immunohistochemistry (IHC-F) Frozen, Immunocytochemistry (ICC), Flow Cytometry (FCM). Flowcytometry: 5-10uL/106cells Immunohistochemistry(Paraffin-embedded Section): 10-50ug/mL Immunohistochemistry(Frozen Section): 10-50ug/mL Immunocytochemistry: 10-50ug/mL Optimal working dilutions must be determined by end users. Researchers should empirically determine the suitability of the FITC Conjugated Goat Anti-mouse IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FITC Conjugated Goat Anti-mouse IgG, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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