Immunohistochemistry (IHC)
(Applications: Electron MicroscopySample: Oyster Visceral MassDetection: 5nm Colloidal Gold"To validate the specificity of the anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) ABIN3042038, 8um paraffin sections of oyster's visceral mass were observed in this study. We compared the level of silver aggregating ability in the presence and absence of ABIN3042038, using commercially available silver enhancement method and SEM-EBSD imaging. We found that the test samples treated with anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) showed distinctive silver aggregates while the primary rabbit anti-SNAP antibody ABIN1573927 was present. These aggregates were not observable in the negative control.")
5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG Secondary Antibody
5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody
Applications
Immunohistochemistry, Immunocytochemistry
Synonyms
5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG; Polyclonal Secondary Antibody; 5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Specificity
This colloidal gold conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse, bovine IgG.
Form/Format
Concentrated, Liquid; 0.1 mg of colloidal gold conjugated specific antibody. the diameter of colloidal gold is 5±1 nm, 0.01 M PBS, 0.01 % Thimerosal, 50% glycerol
Applicable Applications for 5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody
Electron Microscopy (EM), Immunohistochemistry-paraffin/frozen (IHC-Fr/P), Immunocytochemistry (ICC)
Application Notes
EM:1:20-50
IHC-P: 1:50-100
IHC-F: 1:50-100
ICC: 1:50-100
Optimal working dilutions must be determined by end users.
IHC-P: 1:50-100
IHC-F: 1:50-100
ICC: 1:50-100
Optimal working dilutions must be determined by end users.
Immunogen
Rabbit IgG (whole molecule).
Preparation of Diluent Buffer
The conjugate should be diluted for most applications, using 0.01M PBS or TBS, containing 1% BSA.
Preparation of 0.01M TBS:
Add 1.2g Tris, 8.5g NaCl, 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid into 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS:
Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 into 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M TBS:
Add 1.2g Tris, 8.5g NaCl, 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid into 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS:
Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 into 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation and Storage
Store at 4 degree C for one year.
Immunohistochemistry (IHC)
(Applications: Electron MicroscopySample: Oyster Visceral MassDetection: 5nm Colloidal Gold"To validate the specificity of the anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) ABIN3042038, 8um paraffin sections of oyster's visceral mass were observed in this study. We compared the level of silver aggregating ability in the presence and absence of ABIN3042038, using commercially available silver enhancement method and SEM-EBSD imaging. We found that the test samples treated with anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) showed distinctive silver aggregates while the primary rabbit anti-SNAP antibody ABIN1573927 was present. These aggregates were not observable in the negative control.")
Immunohistochemistry (IHC)
(Applications: Electron MicroscopySample: Oyster Visceral MassDetection: 5nm Colloidal Gold"To validate the specificity of the anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) ABIN3042038, 8um paraffin sections of oyster's visceral mass were observed in this study. We compared the level of silver aggregating ability in the presence and absence of ABIN3042038, using commercially available silver enhancement method and SEM-EBSD imaging. We found that the test samples treated with anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) showed distinctive silver aggregates while the primary rabbit anti-SNAP antibody ABIN1573927 was present. These aggregates were not observable in the negative control.")
Related Product Information for 5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody
Description:
This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. The goat anti-rabbit IgG is conjugated to 5 nm colloidal gold and generates electron dense particles that are visible using electron microscopy.
Background:
Immunoglobulin G (IgG) is the most common type of antibody found in the circulation. It is a protein complex composed of four peptide chains—two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. IgG molecules are created and released by plasma B cells. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection.
This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. The goat anti-rabbit IgG is conjugated to 5 nm colloidal gold and generates electron dense particles that are visible using electron microscopy.
Background:
Immunoglobulin G (IgG) is the most common type of antibody found in the circulation. It is a protein complex composed of four peptide chains—two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. IgG molecules are created and released by plasma B cells. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection.
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Product Notes
The 5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG (Catalog #AAA176695) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG can be used in a range of immunoassay formats including, but not limited to, Electron Microscopy (EM), Immunohistochemistry-paraffin/frozen (IHC-Fr/P), Immunocytochemistry (ICC). EM:1:20-50 IHC-P: 1:50-100 IHC-F: 1:50-100 ICC: 1:50-100 Optimal working dilutions must be determined by end users. Researchers should empirically determine the suitability of the 5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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