Protein Synthesis Initiation Factor 4E Recombinant Protein
Protein Synthesis Initiation Factor 4E, Recombinant, Human (eIF 4E, Eukaryotic Translation Initiation Factor)
Purity
Highly Purified90% (SDS-PAGE)
Synonyms
Protein Synthesis Initiation Factor 4E; Recombinant; Human (eIF 4E; Eukaryotic Translation Initiation Factor); Protein Synthesis Initiation Factor 4E recombinant protein
Host
Human recombinant kidney.
Purity/Purification
Highly Purified
90% (SDS-PAGE)
90% (SDS-PAGE)
Form/Format
Supplied as a liquid in 50mM Tris HCl, pH 7.0, b-mercaptoethanol, benzamidine, PMSF, EDTA, 10% glycerol.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Related Product Information for Protein Synthesis Initiation Factor 4E recombinant protein
Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure, thereby mediating the initiation of translation (1,2). eIF4E interacts with eIF4G, which serves as a scaffold protein for the assembly of eIF4E and eIF4A to form the eIF4F complex (2). Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 has been shown to phosphorylate eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites have been identified in mouse Mnk1, Thr197 and Thr202, which are essential for Mnk1 kinase activity (3). The C-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148 and Ser1192 (5). It is known that their phosphorylation is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.
Product Categories/Family for Protein Synthesis Initiation Factor 4E recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
~28kD
NCBI Official Full Name
protein synthesis initiation factor eIF-4E
Uniprot Description
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
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Product Notes
The Protein Synthesis Initiation Factor 4E (Catalog #AAA635559) is a Recombinant Protein produced from Human recombinant kidney. and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Protein Synthesis Initiation Factor 4E, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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