Rabbit PKN1/2 Polyclonal Antibody | anti-PKN1/2 antibody

PKN1/2 (Phospho-T774/T816) polyclonal antibody

Cat. Num. AAA3015089

• Gene Names: PKN1; DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: PKN1/2; Polyclonal Antibody; PKN1/2 (Phospho-T774/T816) polyclonal antibody; Serine/threonine-protein kinase N1; Protease-activated kinase 1; PAK-1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine-threonine protein kinase N; PAK1; PKN; PRK1; PRKCL1; Serine/threonine-protein kinase N2; PKN gamma; Protein kinase C-like 2; Protein-kinase C-related kinase 2; PKN2; PRK2; PRKCL2; anti-PKN1/2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PKN1/2 (Phospho-T774/T816) polyclonal antibody detects endogenous levels of PKN1/2 protein only when phosphorylated at Thr774/Thr816.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PKN1/2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic phosphopeptide derived from human PKN1/2 around the phosphorylation site of Threonine 774/Threonine 816
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of PKN1/2 (Phospho-T774/816) polyclonal antibody at 1:500 dilutionLaneA: HCT116 whole cell lysate)

Related Product Information for anti-PKN1/2 antibody

Protein kinase N (PKN) is a fatty acid-activated serine/threonine kinase whose catalytic domain exhibits homology with that of the PKC family. PKN associates with Rho via its amino terminus, is activated in a GTP-dependent manner and phosphorylates the head-rod domain of neurofilament protein. A second protein, rhophilin, exhibits 40% sequence identity with the amino terminal Rho binding domain. The enzymatic activity of rhophilin has not been demonstrated and it is possible that it acts through the recruitment of cytoskeletal components that initiate a kinase signaling cascade. Citron interacts specifically with active Rho and Rac1 but not Cdc42. Citron exhibits a distinctive protein organization and little homology with the Rho binding domains of PKN and rhophilin.

NCBI and Uniprot Product Information

• NCBI GI #: 47132589
• NCBI GeneID: 5585
• NCBI Accession #: NP_002732.3
• NCBI GenBank Nucleotide #: NM_002741.3
• UniProt Accession #: Q16512; Q16513
• Molecular Weight: 103,932 Da
• NCBI Official Full Name: serine/threonine-protein kinase N1 isoform 2
• NCBI Official Synonym Full Names: protein kinase N1
• NCBI Official Symbol: PKN1
• NCBI Official Synonym Symbols: DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
• NCBI Protein Information: serine/threonine-protein kinase N1; protein kinase C-like 1; protein kinase PKN-alpha; protein kinase C-like PKN; serine-threonine kinase N; protease-activated kinase 1; protein kinase C-related kinase 1; protein-kinase C-related kinase 1; serine-threonin
• UniProt Protein Name: Serine/threonine-protein kinase N1
• UniProt Gene Name: PKN1
• UniProt Synonym Gene Names: PAK1; PKN; PRK1; PRKCL1; PAK-1
• UniProt Entry Name: PKN1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PKN1: an AGC kinase of the PKN family. A PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcriptional regulation. Regulates the cytoskeletal network by phosphorylating intermediate filament proteins such as VIM and NFH, NFL and NFM, inhibiting their polymerization. Phosphorylates Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of H3T11, a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3K9me by JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, impairing their import in the nucleus. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by CASP3 cleavage during apoptosis. Localization to endosomes is mediated via its interaction with RHOB. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.13; AGC group; PKN family

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; midbody; nucleus; endosome; cleavage furrow

Molecular Function: protein kinase C activity; histone binding; histone deacetylase binding; Rac GTPase binding; protein kinase activity; protein serine/threonine kinase activity; protein binding; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; protein kinase C binding; GTP-Rho binding; chromatin binding; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; hyperosmotic response; transcription, DNA-dependent; signal transduction; protein amino acid phosphorylation; activation of JNK activity