PD-L1 / B7-H1 / CD274 recombinant protein

Recombinant Mouse PD-L1 / B7-H1 / CD274 Protein (His tag)

Cat. Num. AAA2547216

• Gene Names: Cd274; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: PD-L1 / B7-H1 / CD274; Recombinant Mouse PD-L1 / B7-H1 / CD274 Protein (His tag); Cd274; B7-H1; PD-L1; Pdcd1l1; Pdcd1lg1; PD-L1 / B7-H1 / CD274 recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 290

• Application Notes: The secreted recombinant mouse CD274 comprises 231 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, it migrates as an approximately 40-45 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Phe 19
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PD-L1 / B7-H1 / CD274 recombinant protein

Background: Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Description: A DNA sequence encoding the mouse CD274 (NP_068693.1) extracellular domain (Met 1-Thr 238) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 11230798
• NCBI GeneID: 60533
• NCBI Accession #: NP_068693.1
• NCBI GenBank Nucleotide #: NM_021893.3
• UniProt Accession #: Q9EP73
• Molecular Weight: 32,780 Da
• NCBI Official Full Name: programmed cell death 1 ligand 1
• NCBI Official Synonym Full Names: CD274 antigen
• NCBI Official Symbol: Cd274
• NCBI Official Synonym Symbols: B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• NCBI Protein Information: programmed cell death 1 ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• UniProt Gene Name: Cd274
• UniProt Synonym Gene Names: B7h1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1

NCBI Description

The protein encoded by this gene is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Mice deficient for this gene display a variety of phenotypes including decreased allogeneic fetal survival rates and severe experimental autoimmune encephalomyelitis. [provided by RefSeq, Sep 2015]

Uniprot Description

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19|19 C1

Cellular Component: cell surface; external side of plasma membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction; immune response; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; negative regulation of T cell proliferation; positive regulation of cell migration; positive regulation of T cell proliferation; response to cytokine stimulus; signal transduction; T cell costimulation