USP46 recombinant protein

USP46 Protein, Human, Mouse, Recombinant

Cat. Num. AAA8121241

• Gene Names: Usp46; AI451644; 1190009E20Rik; 2410018I08Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: USP46; USP46 Protein; Human; Mouse; Recombinant; Human/Mouse USP46 Protein; ubiquitin specific peptidase 46; USP46 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu366

• Species: Human, Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the human/mouse USP46 [(Identical to the mouse USP46 (NP_808229.1)] (Met1-Glu366) was expressed. Human and Mouse USP46 sequences are identical.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for USP46 recombinant protein

Background: USP46 belongs to the peptidase C19 family, USP12/USP46 subfamily. Deubiquitinating enzymes (DUBs) are a large group of proteases which are also commonly referred to as deubiquitinating peptidases, deubiquitinating isopeptidases, deubiquitinases, ubiquitin proteases, ubiquitin hydrolyases, ubiquitin isopeptidases, or Dubs. They regulate ubiquitin-dependent metabolic pathways by cleaving ubiquitin-protein bonds. They also may act as negative and positive regulators of the ubiquitin system. Besides ubiquitin recycling, they are also involved in processing of ubiquitin precursors, in proofreading of protein ubiquitination and in disassembly of inhibitory ubiquitin chains. USP46 is a deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. It may act by mediating the deubiquitination of GAD1/GAD67. USP46 has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have high activity and it is not involved in deubiquitination of monoubiquitinated FANCD2.

References

Joo HY, et al. (2011) Regulation of histone H2A and H2B deubiquitination and Xenopus development by USP12 and USP46. J Biol Chem. 286(9):7190-201.Kushima I, et al. (2010) Association study of ubiquitin-specific peptidase 46 (USP46) with bipolar disorder and schizophrenia in a Japanese population. J Hum Genet. 55(3):133-6.Cohn MA, et al. (2009) UAF1 is a subunit of multiple deubiquitinating enzyme complexes. J Biol Chem. 284(8):5343-51.

NCBI and Uniprot Product Information

• NCBI GI #: 29243896
• NCBI GeneID: 69727
• NCBI Accession #: NP_808229.1
• NCBI GenBank Nucleotide #: NM_177561.3
• UniProt Accession #: P62069; Q3ULU5; Q80V95; Q9H7U4; Q9H9T8
• Molecular Weight: 42,442 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 46
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 46
• NCBI Official Symbol: Usp46
• NCBI Official Synonym Symbols: AI451644; 1190009E20Rik; 2410018I08Rik
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 46; ubiquitin thioesterase 46; deubiquitinating enzyme 46; ubiquitin thiolesterase 46; ubiquitin specific protease 46; ubiquitin-specific-processing protease 46
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 46
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: Usp46
• UniProt Entry Name: UBP46_MOUSE