Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein ELISA Kit | PREX2 elisa kit

Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein ELISA Kit

Cat. Num. AAA764972

• Gene Names: Prex2; Depdc2; P-Rex2; AI316880; AI553603; D430013K02; 6230420N16Rik; C030045D06Rik
• Reactivity: Human
• Synonyms: Phosphatidylinositol 3; 4; 5-trisphosphate-dependent Rac exchanger 2 protein; Human Phosphatidylinositol 3; 5-trisphosphate-dependent Rac exchanger 2 protein ELISA Kit; PREX2/PtdIns (3/4/5)-dependent Rac exchanger 2/DEP domain-containing protein 2/PREX2/DEPDC2; PREX2 elisa kit

• Reactivity: Human
• Sequence Length: 1598

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PREX2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human PREX2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PREX2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PREX2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman PREX2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 183583559
• NCBI GeneID: 109294
• NCBI Accession #: NP_083801.1
• NCBI GenBank Nucleotide #: NM_029525.1
• Molecular Weight: 84,364 Da
• NCBI Official Full Name: phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein isoform 1
• NCBI Official Synonym Full Names: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
• NCBI Official Symbol: Prex2
• NCBI Official Synonym Symbols: Depdc2; P-Rex2; AI316880; AI553603; D430013K02; 6230420N16Rik; C030045D06Rik
• NCBI Protein Information: phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
• UniProt Protein Name: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
• Protein Family: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
• UniProt Gene Name: Prex2
• UniProt Synonym Gene Names: Depdc2; P-Rex2; PtdIns(3,4,5)-dependent Rac exchanger 2
• UniProt Entry Name: PREX2_MOUSE

Uniprot Description

PREX2: Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein- coupled receptors and phosphoinositide 3-kinase. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphatase, regulatory subunit; GEFs, Rac/Rho; GEFs

Cellular Component: plasma membrane

Molecular Function: GTPase activator activity; guanyl-nucleotide exchange factor activity; Rac guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity

Biological Process: adult locomotory behavior; dendrite morphogenesis; G-protein coupled receptor protein signaling pathway; phosphoinositide 3-kinase cascade; positive regulation of GTPase activity; regulation of Rho protein signal transduction