Mouse CD4 Monoclonal Antibody | anti-CD4 antibody

MOUSE ANTI HUMAN CD4

Cat. Num. AAA212066

• Gene Names: CD4; CD4mut
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: CD4; Monoclonal Antibody; MOUSE ANTI HUMAN CD4; anti-CD4 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: RPA-T4
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1 mg/ml (varies by lot)
• Sequence Length: 458

• Applicable Applications for anti-CD4 antibody: Immunohistology Frozen*, Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood ; Immunohistology - Frozen: Minimum Dilution: 1/10; Maximum Dilution: 1/100; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Histology Positive Control Tissue: Human lymphatic tissue; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Alexa Fluor 405 (MBS212066A405))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:FITC (MBS210641))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Biotin (MBS210319))

Testing Data

(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. High power)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:RPE (MBS213570))

Testing Data

(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Medium power)

Testing Data

(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Low power)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Alexa Fluor 488 (MBS212066A488))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Pacific Blue (MBS212066PB))

NCBI and Uniprot Product Information

• NCBI GI #: 10835167
• NCBI GeneID: 920
• NCBI Accession #: NP_000607.1
• NCBI GenBank Nucleotide #: NM_000616.4
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4 isoform 1
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 antigen (p55); CD4 receptor; T-cell surface antigen T4/Leu-3
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; external side of plasma membrane; lipid raft

Molecular Function: protein binding; transmembrane receptor activity; protein homodimerization activity; enzyme binding; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding

Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; defense response to Gram-negative bacterium; signal transduction; induction by virus of cell-cell fusion in host; enzyme linked receptor protein signaling pathway; T cell receptor signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; T cell costimulation; innate immune response; entry into host cell; immune response; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of T cell activation; T cell differentiation

Disease: Okt4 Epitope Deficiency