Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) Standard Curve.)
Mouse Phosphatidylinositol 3, 4, 5-Trisphosphate-Dependent Rac Exchanger 2 Protein (PREX2) ELISA Kit | PREX2 elisa kit
Mouse Phosphatidylinositol 3, 4, 5-Trisphosphate-Dependent Rac Exchanger 2 Protein (PREX2) ELISA Kit
Gene Names
Prex2; Depdc2; P-Rex2; AI316880; AI553603; D430013K02; 6230420N16Rik; C030045D06Rik
Reactivity
Mouse
Synonyms
Phosphatidylinositol 3; 4; 5-Trisphosphate-Dependent Rac Exchanger 2 Protein (PREX2); Mouse Phosphatidylinositol 3; 5-Trisphosphate-Dependent Rac Exchanger 2 Protein (PREX2) ELISA Kit; PREX2; DEP.2; DEPDC2; P-REX2; 6230420N16Rik; DEP domain containing 2; PtdIns(3; 5)-dependent Rac exchanger 2; phosphatidylinositol 3; 5-trisphosphate-dependent RAC exchanger 2; PREX2 elisa kit
Reactivity
Mouse
Specificity
Mouse Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PREX2. No significant cross-reactivity or interference between Mouse PREX2 and analogues was observed.
Sequence Length
1598
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) Standard Curve.)
Related Product Information for PREX2 elisa kit
Description: This Mouse Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2) ELISA Kit employs a two-site sandwich ELISA to quantitate PREX2 in samples. An antibody specific for PREX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PREX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PREX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PREX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Prex2 was abundantly expressed in mouse brain and lung, with lower expression in liver, thymus, and spleen. In brain, highest expression was in cerebellum, with lower expression in frontal cortex, striatum, amygdala and hippocampus. In situ hybridization showed that Prex2 mRNA was highly restricted to cerebellum, with expression in Purkinje cells, including dendrites.Expression of PREX2 in PAE cells induced this 'activated Rac' morphology, which was reduced by inhibition of PI3K signaling and enhanced by PDGF stimulation. PREX2-mediated GTP loading onto RAC was synergistically stimulated by PI3K and G protein beta-1 (GNB1)/gamma-2 (GNG2) subunits. Direct binding of phosphatidylinositol 3,4,5-trisphosphate to PREX2 was sufficient to stimulate PREX2 RAC-guanine nucleotide exchange factor activity.
Background: Prex2 was abundantly expressed in mouse brain and lung, with lower expression in liver, thymus, and spleen. In brain, highest expression was in cerebellum, with lower expression in frontal cortex, striatum, amygdala and hippocampus. In situ hybridization showed that Prex2 mRNA was highly restricted to cerebellum, with expression in Purkinje cells, including dendrites.Expression of PREX2 in PAE cells induced this 'activated Rac' morphology, which was reduced by inhibition of PI3K signaling and enhanced by PDGF stimulation. PREX2-mediated GTP loading onto RAC was synergistically stimulated by PI3K and G protein beta-1 (GNB1)/gamma-2 (GNG2) subunits. Direct binding of phosphatidylinositol 3,4,5-trisphosphate to PREX2 was sufficient to stimulate PREX2 RAC-guanine nucleotide exchange factor activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein isoform 1
NCBI Official Synonym Full Names
phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
NCBI Official Symbol
Prex2
NCBI Official Synonym Symbols
Depdc2; P-Rex2; AI316880; AI553603; D430013K02; 6230420N16Rik; C030045D06Rik
NCBI Protein Information
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
UniProt Protein Name
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
UniProt Gene Name
Prex2
UniProt Synonym Gene Names
Depdc2; P-Rex2; PtdIns(3,4,5)-dependent Rac exchanger 2
UniProt Entry Name
PREX2_MOUSE
Research Articles on PREX2
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Product Notes
The Mouse PREX2 prex2 (Catalog #AAA9717293) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717293 ELISA Kit recognizes Mouse PREX2. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol 3, 4, 5-Trisphosphate-Dependent Rac Exchanger 2 Protein (PREX2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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