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Western Blot (WB) (Anti-ZAP70 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse spleen tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:70 kDa Observed band size:70 kDa)

Rabbit anti-Mouse ZAP70 Polyclonal Antibody | anti-ZAP70 antibody

Anti-ZAP70 Antibody, Rabbit Polyclonal

Reactivity
Mouse
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
ZAP70; Polyclonal Antibody; Anti-ZAP70 Antibody; Rabbit Polyclonal; ZAP70 Antibody; Rabbit PAb; Antigen Affinity Purified; zeta-chain (TCR) associated protein kinase 70kDa; Anti-mrtle Antibody; Anti-mur Antibody; Anti-Srk Antibody; Anti-ZAP-70 Antibody; anti-ZAP70 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse ZAP70
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ZAP70 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the center region of the Mouse ZAP70
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse ZAP70, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ZAP70 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse spleen tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:70 kDa Observed band size:70 kDa)

Western Blot (WB) (Anti-ZAP70 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse spleen tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:70 kDa Observed band size:70 kDa)
Related Product Information for anti-ZAP70 antibody
The ZAP70 (zeta-associated protein of 70 kDa) is expressed in the aggressive form of B-cell chronic lymphocytic leukemia (CLL). ZAP70 plays a critical role for ZAP70 in chemokine-mediated, inside-out signaling to integrins in trisomy. The phosphorylation of ZAP70 activated the downstream NF-kappaB signaling pathway, which is involved in both T cell growth and function. On the other hand, the expression of Zap70 in B-cell malignancies correlates with the severity of the disease. Because of its role in immune-related disorders, Zap70 has become a therapeutic target for the treatment of human diseases.
Product Categories/Family for anti-ZAP70 antibody

NCBI and Uniprot Product Information

UniProt Accession #
UniProt Protein Name
Tyrosine-protein kinase ZAP-70
Protein Family
UniProt Gene Name
Zap70
UniProt Synonym Gene Names
Srk; Zap-70
UniProt Entry Name
ZAP70_MOUSE

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; membrane; cytoplasm; plasma membrane; immunological synapse; intracellular; intercellular junction; cytosol; lipid raft

Molecular Function: transferase activity; protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; receptor binding; protein kinase activity

Biological Process: thymic T cell selection; cell migration; peptidyl-tyrosine phosphorylation; immune system process; protein amino acid autophosphorylation; positive regulation of calcium-mediated signaling; protein amino acid phosphorylation; T cell receptor signaling pathway; negative thymic T cell selection; positive regulation of T cell differentiation; innate immune response; positive regulation of alpha-beta T cell proliferation; immune response; beta selection; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive thymic T cell selection

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Product Notes

The ZAP70 zap70 (Catalog #AAA8103357) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ZAP70 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZAP70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ZAP70 zap70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZAP70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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