Testing Data
Rabbit anti-Rat FMRFamide Antibody | anti-FMRFamide antibody
FMRFamide, Antiserum, Rabbit
Gene Names
FMRFa; CG2346; dFmrf; dFMRF; dFmrfa; dFMRFa; dFMRFa-1; dFMRFa-2; dFMRFa-3; dFMRFa-4; dFMRFa-5; dFMRFa-6; dFMRFa-7; dFMRFa-8; DmelCG2346; DPKQDFMRF-amide; DPKQDFMRFamide; Drm-FMRF-1; Drm-FMRF-2; Drm-FMRF-3; Drm-FMRF-4; Drm-FMRF-5; Drm-FMRFa1; Drm-FMRFa2; Drm-FMRF
Reactivity
Rat
Applications
Immunohistochemistry
Synonyms
FMRFamide; Antiserum; Rabbit; anti-FMRFamide antibody
Host
Rabbit
Reactivity
Rat
Form/Format
Whole Serum (with 0.05% sodium azide) Sent in liquid form.
Applicable Applications for anti-FMRFamide antibody
Immunohistochemistry (IHC)
Application Notes
Immunohistochemistry: 1:200-1:1,000
Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
Immunohistochemistry:
Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehye-picric acid fixative, and 3) 10% sucrose inPBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.
Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.
Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
Immunohistochemistry:
Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehye-picric acid fixative, and 3) 10% sucrose inPBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.
Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.
Immunogen Sequence
FMRFamide
Preparation and Storage
Store frozen. Aliquot as undiluted serum and immediately place at -20ºC. Serum may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6 months at -20ºC. Repeated freeze/thaw cycles compromise the integrity of the antiserum.
Testing Data
Testing Data
Testing Data
(Image below: Labeling for FMRFamide in neuronal processes in frozen sections of rat hypothalamus.)
Testing Data
(Image below: Labeling for FMRFamide in neuronal processes in frozen sections of rat hypothalamus.)
Related Product Information for anti-FMRFamide antibody
First purified from clam ganglia, FMRFamide (Phe-Met-Arg-Phe-NH2) is expressed in both invertebrates and vertebrates. its has been shown to serve various functions in the mammalian nervous system including inhibition of morphine-induced feeding, behavioral effects when administered intrathecally, and to have amnesic effects in rodents. More recently, FMRFamide has been shown to potentiate proton currents and a suggested mode of action is through modulation of ASIC1 and ASIC 3.
Product Categories/Family for anti-FMRFamide antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
FMRFamide
NCBI Official Synonym Full Names
FMRFamide
NCBI Official Symbol
FMRFa
NCBI Official Synonym Symbols
CG2346; dFmrf; dFMRF; dFmrfa; dFMRFa; dFMRFa-1; dFMRFa-2; dFMRFa-3; dFMRFa-4; dFMRFa-5; dFMRFa-6; dFMRFa-7; dFMRFa-8; DmelCG2346; DPKQDFMRF-amide; DPKQDFMRFamide; Drm-FMRF-1; Drm-FMRF-2; Drm-FMRF-3; Drm-FMRF-4; Drm-FMRF-5; Drm-FMRFa1; Drm-FMRFa2; Drm-FMRF
NCBI Protein Information
CG2346 gene product from transcript CG2346-RA
Protein Family
Research Articles on FMRFamide
Similar Products
Product Notes
The FMRFamide (Catalog #AAA555213) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FMRFamide, Antiserum, Rabbit reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FMRFamide can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Immunohistochemistry: 1:200-1:1,000 Dilutions listed as a recommendation. Optimal dilution should be determined by investigator. Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehye-picric acid fixative, and 3) 10% sucrose inPBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Researchers should empirically determine the suitability of the FMRFamide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FMRFamide, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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