Mouse anti-Human, mouse Zap70 Monoclonal Antibody | anti-Zap70 antibody

Zap70 Antibody

Cat. Num. AAA9200657

• Gene Names: Zap70; Srk; mur; mrtle; ZAP-70
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA
• Purity: Purified Mouse Monoclonal Antibody (Mab)
• Synonyms: Zap70; Monoclonal Antibody; Zap70 Antibody; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; Srk; Zap-70; anti-Zap70 antibody

• Host: Mouse
• Reactivity: Human, mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1484CT290.68.62
• Specificity: This Zap70 antibody is generated from a mouse immunized with a recombinant protein.
• Purity/Purification: Purified Mouse Monoclonal Antibody (Mab)
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 312

• Applicable Applications for anti-Zap70 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Zap70 Antibody at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: MOLT-4 whole cell lysatesLane 3: mouse thymus lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Zap70 antibody

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

Product Categories/Family for anti-Zap70 antibody

Crown Antibodies

References

Gauen L.K.T.,et al.Mol. Cell. Biol. 14:3729-3741(1994).
Wiest D.L.,et al.Immunity 6:663-671(1997).
Kuroyama H.,et al.Biochem. Biophys. Res. Commun. 315:935-941(2004).
Ikeda T.,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Carninci P.,et al.Science 309:1559-1563(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 576067923
• NCBI GeneID: 22637
• NCBI Accession #: NP_001276541.1
• NCBI GenBank Nucleotide #: NM_001289612.1
• UniProt Accession #: P43404; P97455; Q80VV2; Q8CHJ3
• Molecular Weight: 70112
• NCBI Official Full Name: tyrosine-protein kinase ZAP-70 TZK
• NCBI Official Synonym Full Names: zeta-chain (TCR) associated protein kinase
• NCBI Official Symbol: Zap70
• NCBI Official Synonym Symbols: Srk; mur; mrtle; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Zap70
• UniProt Synonym Gene Names: Srk; Zap-70
• UniProt Entry Name: ZAP70_MOUSE

NCBI Description

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; membrane; cytoplasm; plasma membrane; immunological synapse; intercellular junction; intracellular; cytosol; lipid raft

Molecular Function: transferase activity; protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; kinase activity; ATP binding; receptor binding; protein kinase activity

Biological Process: thymic T cell selection; cell migration; peptidyl-tyrosine phosphorylation; immune system process; protein amino acid autophosphorylation; positive regulation of calcium-mediated signaling; protein amino acid phosphorylation; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of T cell differentiation; innate immune response; beta selection; positive regulation of alpha-beta T cell proliferation; immune response; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive thymic T cell selection