Flow Cytometry (FC)
Mouse anti-Human ZAP-70 (TCR zeta) Monoclonal Antibody | anti-Zap70 antibody
Mouse Anti-Human ZAP-70-BIOT
Flow Cytometry (FC)
Flow Cytometry (FC)
3. Van Oers, N.S., and A. Weiss. 1995. Semin. Immunol. 7:227.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Uniprot Description
Function: Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. Ref.3
Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure: Interacts with CBL. Interacts with SHB. Interacts with FCRL3
By similarity. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with SLA and SLA2 when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with CBLB and DEF6. Interacts (via SH2 domains) with RHOH. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10
Subcellular location: Cytoplasm. Cell membrane. Note: After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. Ref.3 Ref.10
Tissue specificity: Isoform 1 and isoform 2 are expressed in thymus, sleen and lymph nodes. Ref.3
Developmental stage: Isoform 2 is expressed in develoing thymocytes from the CD44+CD25- stage up to mature T cells. Isoform 1 is not expressed in thymocytes at the CD44+CD25- or CD44+CD25+ stages. Ref.3
Domain: The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH. Ref.10
Post-translational modification: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-318 phosphorylation is essential for full activity
Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.Contains 1 protein kinase domain.Contains 2 SH2 domains.
Research Articles on Zap70
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Product Notes
The Zap70 zap70 (Catalog #AAA670111) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human ZAP-70-BIOT reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZAP-70 (TCR zeta) can be used in a range of immunoassay formats including, but not limited to, ELISA; Flow Cytometry. FITC and BIOT conjugates < = 1 ug/106 cells. Researchers should empirically determine the suitability of the Zap70 zap70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZAP-70 (TCR zeta), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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