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Western Blot (WB) (All lanes : Anti-Yes1 Antibody (Center) at 1:2000 dilutionLane 1: mouse lung lysatesLane 2: L6 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 61 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Mouse, rat Yes1 Polyclonal Antibody | anti-Yes1 antibody

Mouse Yes1 Antibody (Center)

Gene Names
Yes1; Yes; p61-Yes
Reactivity
Mouse, rat
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Yes1; Polyclonal Antibody; Mouse Yes1 Antibody (Center); Tyrosine-protein kinase Yes; Proto-oncogene c-Yes; p61-Yes; Yes; anti-Yes1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Yes1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 148-182 amino acids from the Central region of Mouse Yes1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
541
Applicable Applications for anti-Yes1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:2000
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Yes1 Antibody (Center) at 1:2000 dilutionLane 1: mouse lung lysatesLane 2: L6 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 61 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-Yes1 Antibody (Center) at 1:2000 dilutionLane 1: mouse lung lysatesLane 2: L6 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 61 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Yes1 antibody
Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis (By similarity).
References
Klages S.,et al.Oncogene 8:713-719(1993).
Hebert B.,et al.Gene 143:257-260(1994).
Courtneidge S.A.,et al.EMBO J. 12:943-950(1993).
Stein P.L.,et al.Genes Dev. 8:1999-2007(1994).
Ariki M.,et al.J. Biochem. 121:104-111(1997).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60630
NCBI Official Full Name
tyrosine-protein kinase Yes
NCBI Official Synonym Full Names
Yamaguchi sarcoma viral (v-yes) oncogene homolog 1
NCBI Official Symbol
Yes1
NCBI Official Synonym Symbols
Yes; p61-Yes
NCBI Protein Information
tyrosine-protein kinase Yes
UniProt Protein Name
Tyrosine-protein kinase Yes
Protein Family
UniProt Gene Name
Yes1
UniProt Synonym Gene Names
Yes
UniProt Entry Name
YES_MOUSE

Uniprot Description

Yes: a proto-oncogenic cytosolic tyrosine kinase of the Src family. Expressed at high levels in adult neurons, spermatozoa, platelets, and epithelial cells.

Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; Oncoprotein; Protein kinase, TK; TK group; Src family

Cellular Component: Golgi apparatus; extrinsic to internal side of plasma membrane; focal adhesion; cytoskeleton; membrane; cytoplasm; plasma membrane; actin filament

Molecular Function: transferase activity; protein binding; enzyme binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; epidermal growth factor receptor binding; receptor binding; protein kinase activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; glucose transport; cell differentiation; phosphorylation; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation

Research Articles on Yes1

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Product Notes

The Yes1 yes1 (Catalog #AAA9209526) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Yes1 Antibody (Center) reacts with Mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Yes1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the Yes1 yes1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Yes1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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