Immunohistochemistry (IHC)
(Immunochemical staining of human CHUK in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human CHUK Polyclonal Antibody | anti-CHUK antibody
Anti-CHUK Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
CHUK; Polyclonal Antibody; Anti-CHUK Antibody; Rabbit Polyclonal; CHUK Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-IKBKA Antibody; Anti-IKK-alpha Antibody; Anti-IKK1 Antibody; Anti-IKKA Antibody; Anti-NFKBIKA Antibody; Anti-TCF16 Antibody; anti-CHUK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CHUK
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-CHUK antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human CHUK fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human CHUK fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human CHUK in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CHUK in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-CHUK antibody
CHUK (conserved helix-loop-helix ubiquitous kinase) is ubiquitously expressed in diverse types of established cell lines, whereas it is differentially expressed in normal murine tissues. The structural features of the CHUK polypeptide suggest that its putative kinase activity may be targetted to H-L-H and/or leucine zipper transcription factors. Alternatively, the dual amphipathic a-helices may serve to control its intrinsic kinase activity by interactions with other cellular factors. CHUK may provide new insights into the regulated transmission of cytoplasmic signals to specific nuclear factors manifesting rapid alterations in patterns of cellular gene expression. The non-canonical NF-kappaB activating kinase IKKalpha, encoded by CHUK, modulates pro-or anti-inflammatory responses, cellular survival, and cellular differentiation. CHUK has an essential role in the development of the skin epidermis and its derivatives, along with various other morphogenetic events.
References
Olivotto E, et al. (2013) Ikkalpha/chuk regulates extracellular matrix remodeling independent of its kinase activity to facilitate articular chondrocyte differentiation. PLoS One 8 (9): e73024.Lahtela J, et al. (2010) Mutant chuk and severe fetal encasement malformation. N Engl J Med 363 (17): 1631-1637.Culley KL, et al. (2019) Inducible knockout of chuk/ikkalpha in adult chondrocytes reduces progression of cartilage degradation in a surgical model of osteoarthritis. Sci Rep 9 (1): 8905.Connelly MA, et al. (1995) Chuk, a new member of the helix-loop-helix and leucine zipper families of interacting proteins, contains a serine-threonine kinase catalytic domain. Cell Mol Biol Res 41 (6): 537-549.
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Product Notes
The CHUK (Catalog #AAA8108335) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CHUK Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHUK can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the CHUK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHUK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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