Mouse anti-Human YES1 Monoclonal Antibody | anti-YES1 antibody

YES1 Antibody

Cat. Num. AAA9216879

• Gene Names: YES1; Yes; c-yes; HsT441; P61-YES
• Reactivity: Human
• Applications: Western Blot
• Synonyms: YES1; Monoclonal Antibody; YES1 Antibody; Purified Mouse Monoclonal Antibody (Mab); Tyrosine-protein kinase Yes; 2.7.10.2; Proto-oncogene c-Yes; p61-Yes; YES; anti-YES1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Sequence Length: 543

• Applicable Applications for anti-YES1 antibody: Western Blot (WB)
• Application Notes: WB~~1:4000
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-YES1 Antibody at 1:4000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-YES1 antibody

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis.

Product Categories/Family for anti-YES1 antibody

Crown Antibodies; Cancer; Neuroscience; Signal Transduction

References

Sukegawa J.,et al.Mol. Cell. Biol. 7:41-47(1987). Nusbaum C.,et al.Nature 437:551-555(2005). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Sugawara K.,et al.Br. J. Cancer 63:508-513(1991). Krueger J.,et al.Oncogene 6:933-940(1991).

NCBI and Uniprot Product Information

• NCBI GI #: 4885661
• NCBI GeneID: 7525
• NCBI Accession #: NP_005424.1
• NCBI GenBank Nucleotide #: NM_005433.3
• UniProt Accession #: P07947; A6NLB3; D3DUH1
• Molecular Weight: 60,801 Da
• NCBI Official Full Name: tyrosine-protein kinase Yes
• NCBI Official Synonym Full Names: YES proto-oncogene 1, Src family tyrosine kinase
• NCBI Official Symbol: YES1
• NCBI Official Synonym Symbols: Yes; c-yes; HsT441; P61-YES
• NCBI Protein Information: tyrosine-protein kinase Yes
• UniProt Protein Name: Tyrosine-protein kinase Yes
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: YES1
• UniProt Synonym Gene Names: YES
• UniProt Entry Name: YES_HUMAN

NCBI Description

This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

Yes: a proto-oncogenic cytosolic tyrosine kinase of the Src family. Expressed at high levels in adult neurons, spermatozoa, platelets, and epithelial cells.

Protein type: EC 2.7.10.2; Protein kinase, TK; Oncoprotein; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Src family

Chromosomal Location of Human Ortholog: 18p11.31-p11.21

Cellular Component: actin filament; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; focal adhesion; Golgi apparatus; microtubule organizing center; plasma membrane

Molecular Function: ATP binding; enzyme binding; epidermal growth factor receptor binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein-tyrosine kinase activity; receptor binding

Biological Process: axon guidance; blood coagulation; cell differentiation; ephrin receptor signaling pathway; glucose transport; innate immune response; leukocyte migration; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein modification process; regulation of cell proliferation; regulation of vascular permeability; T cell costimulation; transmembrane receptor protein tyrosine kinase signaling pathway