Rabbit Lyn Polyclonal Antibody | anti-Lyn antibody

Mouse Lyn Antibody (Center)

Cat. Num. AAA9213874

• Gene Names: Lyn; Hck-2; p53Lyn; p56Lyn; AA407514
• Reactivity: Human, mouse, rat
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Lyn; Polyclonal Antibody; Mouse Lyn Antibody (Center); Tyrosine-protein kinase Lyn; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; p53Lyn; p56Lyn; anti-Lyn antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Lyn antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 229-263 amino acids from the Central region of human Mouse Lyn.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 491

• Applicable Applications for anti-Lyn antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Lyn Antibody (Center) at 1:2000 dilutionLane 1: mouse kidney lysatesLane 2: mouse heart lysatesLane 3: mouse ovary lysatesLane 4: mouse skeletal muscle lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded M.spleen section using Mouse Lyn Antibody (Center). NA was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Western Blot (WB)

(Western blot analysis of lysates from Raji cell line and rat spleen tissue lysate(from left to right), using Mouse Lyn Antibody (Center). MBS9213874 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Related Product Information for anti-Lyn antibody

Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. Functions primarily as negative regulator, but can also function as activator, depending on the context. Required for the initiation of the B-cell response, but also for its down- regulation and termination. Plays an important role in the regulation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance. Acts downstream of several immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Plays a role in the inflammatory response to bacterial lipopolysaccharide. Mediates the responses to cytokines and growth factors in hematopoietic progenitors, platelets, erythrocytes, and in mature myeloid cells, such as dendritic cells, neutrophils and eosinophils. Acts downstream of EPOR, KIT, MPL, the chemokine receptor CXCR4, as well as the receptors for IL3, IL5 and CSF2. Plays an important role in integrin signaling. Regulates cell proliferation, survival, differentiation, migration, adhesion, degranulation, and cytokine release. Down- regulates signaling pathways by phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIM), that then serve as binding sites for phosphatases, such as PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1, that modulate signaling by dephosphorylation of kinases and their substrates. Phosphorylates LIME1 in response to CD22 activation. Phosphorylates BTK, CBL, CD5, CD19, CD72, CD79A, CD79B, CSF2RB, DOK1, HCLS1, LILRB3/PIR-B, MS4A2/FCER1B, PTK2B/PYK2, SYK and TEC. Promotes phosphorylation of SIRPA, PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1. Required for rapid phosphorylation of FER in response to FCER1 activation. Mediates KIT phosphorylation. Acts as an effector of EPOR (erythropoietin receptor) in controlling KIT expression and may play a role in erythroid differentiation during the switch between proliferation and maturation. Depending on the context, activates or inhibits several signaling cascades. Regulates phosphatidylinositol 3- kinase activity and AKT1 activation. Regulates activation of the MAP kinase signaling cascade, including activation of MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1 and MAPK9/JNK2. Mediates activation of STAT5A and/or STAT5B. Phosphorylates LPXN on 'Tyr- 72'.

Product Categories/Family for anti-Lyn antibody

Signal Transduction

References

Stanley E.,et al.Mol. Cell. Biol. 11:3399-3406(1991).
Yi T.,et al.Mol. Cell. Biol. 11:2391-2398(1991).
Wilks A.F.,et al.Gene 85:67-74(1989).
Yamanashi Y.,et al.Science 251:192-194(1991).
Clark M.R.,et al.EMBO J. 13:1911-1919(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 161760636
• NCBI GeneID: 17096
• NCBI Accession #: NP_001104566.1
• NCBI GenBank Nucleotide #: NM_001111096.1
• UniProt Accession #: P25911; Q62127
• Molecular Weight: 58812
• NCBI Official Full Name: tyrosine-protein kinase Lyn isoform A
• NCBI Official Synonym Full Names: Yamaguchi sarcoma viral (v-yes-1) oncogene homolog
• NCBI Official Symbol: Lyn
• NCBI Official Synonym Symbols: Hck-2; p53Lyn; p56Lyn; AA407514
• NCBI Protein Information: tyrosine-protein kinase Lyn
• UniProt Protein Name: Tyrosine-protein kinase Lyn
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Lyn
• UniProt Entry Name: LYN_MOUSE

Uniprot Description

LYN: a tyrosine kinase of the Src family that plays a role in B cell antigen-receptor signaling.

Protein type: Protein kinase, TK; Kinase, protein; Oncoprotein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Src family

Cellular Component: Golgi apparatus; mast cell granule; cell; postsynaptic density; mitochondrial intermembrane space; lipid raft; mitochondrial crista; extrinsic to internal side of plasma membrane; cell-cell adherens junction; membrane; perinuclear region of cytoplasm; cytoplasm; mitochondrial membrane; plasma membrane; nucleus

Molecular Function: gamma-tubulin binding; erythropoietin receptor binding; platelet-derived growth factor receptor binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphoprotein binding; glycosphingolipid binding; protein kinase activity; transferase activity; integrin binding; protein binding; enzyme binding; ubiquitin protein ligase binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; protein complex binding; kinase activity; SH3 domain binding; ATP binding; receptor binding

Biological Process: regulation of mast cell degranulation; negative regulation of MAP kinase activity; central nervous system development; positive regulation of B cell receptor signaling pathway; protein amino acid phosphorylation; regulation of B cell apoptosis; B cell receptor signaling pathway; erythrocyte differentiation; tolerance induction to self antigen; neurite development; hemoglobin biosynthetic process; immune response-regulating cell surface receptor signaling pathway; regulation of erythrocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; histamine secretion by mast cell; cytokine secretion; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of mast cell activation; regulation of cytokine secretion; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; response to hormone stimulus; negative regulation of B cell proliferation; chemotaxis; signal transduction; regulation of cytokine production; negative regulation of cell proliferation; regulation of cell adhesion mediated by integrin; platelet degranulation; B cell homeostasis; negative regulation of protein amino acid phosphorylation; positive regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; hemopoiesis; Fc receptor mediated inhibitory signaling pathway; Fc receptor mediated stimulatory signaling pathway; inflammatory response; regulation of B cell receptor signaling pathway; immune system process; negative regulation of myeloid leukocyte differentiation; negative regulation of toll-like receptor 2 signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of cell motility; regulation of release of sequestered calcium ion into cytosol; positive regulation of tyrosine phosphorylation of STAT protein; regulation of inflammatory response; negative regulation of toll-like receptor 4 signaling pathway; innate immune response; phosphorylation; response to DNA damage stimulus; positive regulation of phosphorylation; positive regulation of cell migration