Rabbit anti-Rat Arg1 Polyclonal Antibody | anti-Arg1 antibody

Arg1, CT (Arg1, Arginase-1, Liver-type arginase, Type I arginase) (PE)

Cat. Num. AAA6274796

• Reactivity: Rat
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Arg1; Polyclonal Antibody; CT (Arg1; Arginase-1; Liver-type arginase; Type I arginase) (PE); Type I arginase; anti-Arg1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Arg1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Rat Arg1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-318 amino acids from the C-terminal region of human Rat Arg1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Rat Arg1 Antibody (C-term) western blot analysis in rat liver tissue lysates (35ug/lane).This demonstrates the Rat Arg1 antibody detected the Rat Arg1 protein (arrow).)

Related Product Information for anti-Arg1 antibody

catalyzes the hydrolysis of arginine to ornithine and urea in arginine metabolism; regulates nitric oxide production [RGD].

Product Categories/Family for anti-Arg1 antibody

Antibodies; Enzymes

References

Warnken, M., et al. Naunyn Schmiedebergs Arch. Pharmacol. 381(4):297-304(2010). Zhu, W., et al. Am. J. Physiol., Cell Physiol. 298 (4), C952-C960 (2010): Bagnost, T., et al. Hypertens. Res. 32(12):1130-1135(2009). Kim, J.H., et al. J. Appl. Physiol. 107(4):1249-1257(2009). Deng, K., et al. J. Neurosci. 29(30):9545-9552(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 166091458
• NCBI GeneID: 29221
• NCBI Accession #: NP_058830.2
• NCBI GenBank Nucleotide #: NM_017134.3
• UniProt Accession #: P07824; Q5BK93
• Molecular Weight: 34,973 Da
• NCBI Official Full Name: arginase-1
• NCBI Official Synonym Full Names: arginase 1
• NCBI Official Symbol: Arg1
• NCBI Protein Information: arginase-1
• UniProt Protein Name: Arginase-1
• Protein Family: Arginase
• UniProt Gene Name: Arg1
• UniProt Entry Name: ARGI1_RAT

NCBI Description

catalyzes the hydrolysis of arginine to ornithine and urea in arginine metabolism; regulates nitric oxide production [RGD, Feb 2006]

Uniprot Description

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.5.3.1; Amino Acid Metabolism - arginine and proline

Cellular Component: extracellular space; mitochondrial outer membrane; neuron projection; cell soma; cytoplasm; nucleus

Molecular Function: manganese ion binding; arginase activity

Biological Process: mammary gland involution; response to peptide hormone stimulus; maternal process involved in pregnancy; response to lipopolysaccharide; female pregnancy; response to vitamin A; response to selenium ion; response to vitamin E; response to methylmercury; response to wounding; response to axon injury; collagen biosynthetic process; aging; response to drug; response to herbicide; liver development; response to amino acid stimulus; response to manganese ion; response to cadmium ion; arginine metabolic process; response to zinc ion; response to steroid hormone stimulus; arginine catabolic process to ornithine; positive regulation of endothelial cell proliferation; lung development; response to amine stimulus; urea cycle