Rabbit MAPRE2 Polyclonal Antibody | anti-MAPRE2 antibody

MAPRE2 Polyclonal Antibody

Cat. Num. AAA3006909

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MAPRE2; Polyclonal Antibody; MAPRE2 Polyclonal Antibody; Microtubule-associated protein RP/EB family member 2; APC-binding protein EB2; End-binding protein 2; EB2; RP1; anti-MAPRE2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MAPRE2 polyclonal antibody detects endogenous levels of MAPRE2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 327

• Applicable Applications for anti-MAPRE2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MAPRE2
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MAPRE2 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysate Lane3:H9C2 whole cell lysate)

Related Product Information for anti-MAPRE2 antibody

APC (adenomatous polyposis coli) is a tumor suppressor gene that is frequently mutated in colorectal cancers and is one of the earliest indicators of colorectal carcinogenesis. APC is widely expressed and is largely similar to the related brain-specific homolog APCL. These proteins both associate with beta-catenin and functionally regulate the levels of intracellular beta-catenin. Additionally, these homologs interact with the microtubule cytoskeletal protein EB1 to regulate cell cycle progression or growth control. EB1 family proteins (EB1, RP1 (EB2) and EB3) interact with cytoplasmic microtubules ininterphase cells, with mitotic spindles, and with the APC tumor suppressor gene. The functional inactivation of the APC gene product is a key event in colorectal tumorigenesis. RP1 is localized in the plus ends of microtubule networks in the presence or absence of APC. The gene which encodes RP1 maps to human chromosome 18q12.1.

NCBI and Uniprot Product Information

• NCBI GI #: 10346135
• NCBI Accession #: NP_055083.1
• NCBI GenBank Nucleotide #: NP_055083.1
• UniProt Accession #: Q15555
• Molecular Weight: ~ 37kDa
• NCBI Official Full Name: microtubule-associated protein RP/EB family member 2 isoform 1
• UniProt Protein Name: Microtubule-associated protein RP/EB family member 2
• Protein Family: Microtubule-associated protein RP/EB family
• UniProt Gene Name: MAPRE2
• UniProt Synonym Gene Names: RP1; EB2
• UniProt Entry Name: MARE2_HUMAN

Uniprot Description

RP1: shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. Its function is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: microtubule cytoskeleton; cytoplasm

Molecular Function: microtubule binding

Biological Process: cell proliferation; mitosis; cell division; signal transduction