Rabbit TJP3 Polyclonal Antibody | anti-TJP3 antibody

TJP3 Polyclonal Antibody

Cat. Num. AAA3006496

• Gene Names: TJP3; ZO3; ZO-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TJP3; Polyclonal Antibody; TJP3 Polyclonal Antibody; Tight junction protein ZO-3; Tight junction protein 3; Zona occludens protein 3; Zonula occludens protein 3; ZO3; anti-TJP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TJP3 polyclonal antibody detects endogenous levels of TJP3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 919

• Applicable Applications for anti-TJP3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human TJP3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TJP3 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)

Related Product Information for anti-TJP3 antibody

Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane-associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines (8). ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.

NCBI and Uniprot Product Information

• NCBI GI #: 597439632
• NCBI GeneID: 27134
• UniProt Accession #: O95049
• Molecular Weight: ~ 110kDa
• NCBI Official Full Name: Tight junction protein ZO-3
• NCBI Official Synonym Full Names: tight junction protein 3
• NCBI Official Symbol: TJP3
• NCBI Official Synonym Symbols: ZO3; ZO-3
• NCBI Protein Information: tight junction protein ZO-3
• UniProt Protein Name: Tight junction protein ZO-3
• Protein Family: Tight junction protein
• UniProt Gene Name: TJP3
• UniProt Synonym Gene Names: ZO3
• UniProt Entry Name: ZO3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012]

Uniprot Description

ZO3: Interacts with occludin, claudins and ZO-1. Interacts with INADL. Interacts with UBN1. Interacts with FASLG. Belongs to the MAGUK family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; tight junction; apical plasma membrane; cell junction; nucleus

Molecular Function: protein binding