Rabbit anti-Human, Mouse Wee2 Polyclonal Antibody | anti-Wee2 antibody

Wee2, ID (Wee2, Wee1b, Wee1-like protein kinase 2, Wee1-like protein kinase 1B, Wee1B kinase) (APC)

Cat. Num. AAA6363523

• Gene Names: Wee2; Wee1B; Gm1065
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Wee2; Polyclonal Antibody; ID (Wee2; Wee1b; Wee1-like protein kinase 2; Wee1-like protein kinase 1B; Wee1B kinase) (APC); Wee1B kinase; anti-Wee2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Wee2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Wee2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 363-393 amino acids from the Central region of Mouse Wee2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Wee2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Wee2 antibody detected the Wee2 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Wee2 Antibody (Center) (AP14472c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse ovarian tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Wee2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Wee2 antibody

Phosphorylates and inhibits CDK1. May act as a negative regulator of entry into mitosis (G2 to M transition).

Product Categories/Family for anti-Wee2 antibody

Antibodies; Protein Kinases

References

Oh, J.S., et al. J. Cell Biol. 188(2):199-207(2010). Joshi, S., et al. BMC Dev. Biol. 7, 67 (2007): Han, S.J., et al. Curr. Biol. 15(18):1670-1676(2005). Nakanishi, M., et al. Genes Cells 5(10):839-847(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 171846235
• NCBI GeneID: 381759
• NCBI Accession #: NP_958758.2
• NCBI GenBank Nucleotide #: NM_201370.2
• UniProt Accession #: Q66JT0; Q4U4S4; Q7TPV9
• Molecular Weight: 62,343 Da
• NCBI Official Full Name: wee1-like protein kinase 2
• NCBI Official Synonym Full Names: WEE1 homolog 2 (S. pombe)
• NCBI Official Symbol: Wee2
• NCBI Official Synonym Symbols: Wee1B; Gm1065
• NCBI Protein Information: wee1-like protein kinase 2
• UniProt Protein Name: Wee1-like protein kinase 2
• Protein Family: Wee1-like protein kinase
• UniProt Gene Name: Wee2
• UniProt Synonym Gene Names: Wee1b; mWee1B

Uniprot Description

WEE1B: a protein kinase of the WEE family expressed only in oocytes. Downregulation by RNAi causes a leaky meiotic arrest. The inhibitory effects of the kinase are enhanced when Ser15 is phosphorylated. Appears to be a key MPF inhibitory kinase in mouse oocytes and is required for maintaining meiotic arrest.

Protein type: EC 2.7.10.2; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); WEE family; WEE1 subfamily

Chromosomal Location of Human Ortholog: 6|6 B1

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: ATP binding; kinase activity; magnesium ion binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein kinase activity; protein tyrosine kinase activity; transferase activity

Biological Process: female meiotic nuclear division; female pronucleus assembly; meiotic cell cycle; mitotic cell cycle; negative regulation of cyclin-dependent protein serine/threonine kinase activity; negative regulation of oocyte maturation; phosphorylation; protein amino acid phosphorylation