Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see the related technical datasheet.)
WEE2 recombinant protein
WEE2, Active
Gene Names
Wee2; Wee1B; Gm1065
Applications
Western Blot
Synonyms
WEE2; Active; WEE1B; WEE2 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Applicable Applications for WEE2 recombinant protein
Kinase Assay, Western Blot (WB)
Tag
GST
Species
Mouse
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot targets into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see the related technical datasheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see the related technical datasheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see the related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see the related technical data sheet.)
Related Product Information for WEE2 recombinant protein
Full-length recombinant mouse WEE2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC052883.
WEE2, also known as WEE1B, is a dual-specificity kinases that uniquely function to phosphorylate specific Tyr/Thr residues on CDKs. It belongs to WEE1 family that also includes WEE1 homolog 1 (WEE1, also designated as somatic WEE1A) and PKMYT1 (previously known as MYT1). It is conserved from yeast to humans and has been shown to suppress CDK1 activity by phosphorylating its inhibitory sites. A key M phase-promoting factor (MPF) inhibitory kinase in oocytes and is required for maintaining meiotic arrest.
WEE2, also known as WEE1B, is a dual-specificity kinases that uniquely function to phosphorylate specific Tyr/Thr residues on CDKs. It belongs to WEE1 family that also includes WEE1 homolog 1 (WEE1, also designated as somatic WEE1A) and PKMYT1 (previously known as MYT1). It is conserved from yeast to humans and has been shown to suppress CDK1 activity by phosphorylating its inhibitory sites. A key M phase-promoting factor (MPF) inhibitory kinase in oocytes and is required for maintaining meiotic arrest.
Product Categories/Family for WEE2 recombinant protein
References
1. Leise W 3rd, et al: Multiple Cdk1 inhibitory kinases regulate the cell cycle during development. Dev Biol. 2002 Sep 1;249(1):156-73.
2. Liu C, et al: Ser 15 of WEE1B is a potential PKA phosphorylation target in G2/M transition in one-cell stage mouse embryos. Mol Med Rep. 7(6):1929-37, 2013.
2. Liu C, et al: Ser 15 of WEE1B is a potential PKA phosphorylation target in G2/M transition in one-cell stage mouse embryos. Mol Med Rep. 7(6):1929-37, 2013.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
96 kDa
NCBI Official Full Name
Mus musculus WEE1 homolog 2 (S. pombe), mRNA
NCBI Official Synonym Full Names
WEE1 homolog 2 (S. pombe)
NCBI Official Symbol
Wee2
NCBI Official Synonym Symbols
Wee1B; Gm1065
NCBI Protein Information
wee1-like protein kinase 2
Protein Family
Research Articles on WEE2
Similar Products
Product Notes
The WEE2 (Catalog #AAA517790) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's WEE2 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the WEE2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WEE2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
